Bierbower Sonya M, Shapiro Mark S
Department of Physiology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
Methods Mol Biol. 2013;998:209-16. doi: 10.1007/978-1-62703-351-0_16.
Understanding the molecular mechanisms of protein-protein interactions at the cell surface of living cells is fundamental to identifying the nature of cellular processes. Here, we discuss how fluorescence-based approaches have been successfully developed to visualize protein-protein interactions in living cells. Förster resonance energy transfer (FRET) is unique in generating fluorescence signals between proteins that are highly spatially sensitive. Furthermore, total internal reflectance fluorescence (TIRF) microscopy combined with FRET is a robust technique used to assay protein/protein interactions and the functionality of proteins assembled at the cell surface membrane.
了解活细胞表面蛋白质 - 蛋白质相互作用的分子机制是确定细胞过程本质的基础。在这里，我们讨论了基于荧光的方法是如何成功开发用于可视化活细胞中蛋白质 - 蛋白质相互作用的。Förster共振能量转移（FRET）在产生高度空间敏感的蛋白质之间的荧光信号方面具有独特性。此外，全内反射荧光（TIRF）显微镜与FRET相结合是一种用于检测蛋白质/蛋白质相互作用以及在细胞表面膜上组装的蛋白质功能的强大技术。
