Tsunoda H, Abe S, Sakuma Y, Katayama S, Katayama K
Inflammation and Allergy Research Unit, Eisai Research Laboratories, Ibaraki, Japan.
Prostaglandins Leukot Essent Fatty Acids. 1990 Apr;39(4):291-4. doi: 10.1016/0952-3278(90)90008-9.
The role of leukotrienes (LTs) in the pathogenesis of platelet-activating factor (PAF)-induced death in mice was reinvestigated, since previously reported results are in conflict. A novel 5-lipoxygenase inhibitor, E6080, and a leukotriene antagonist, LY17883, protected mice from PAF-induced death in a dose-dependent manner, while the well-known 5-lipoxygenase inhibitor, AA861, was less effective than E6080. After the intravenous injection of PAF in mice, immunoreactive leukotriene C4 (i-LTC4), which was co-eluted with authentic LTC4 in HPLC, was significantly increased in bronchoalveolar lavage fluid (BALF). Oral administration of E6080 suppressed the increase in i-LTCM4. The results suggest that LTs may play an important role in PAF-induced lethality in mice.
鉴于先前报道的结果相互矛盾，对白三烯（LTs）在血小板活化因子（PAF）诱导的小鼠死亡发病机制中的作用进行了重新研究。一种新型5-脂氧合酶抑制剂E6080和一种白三烯拮抗剂LY17883以剂量依赖性方式保护小鼠免受PAF诱导的死亡，而著名的5-脂氧合酶抑制剂AA861的效果不如E6080。给小鼠静脉注射PAF后，支气管肺泡灌洗液（BALF）中与真实LTC4在高效液相色谱中共洗脱的免疫反应性白三烯C4（i-LTC4）显著增加。口服E6080可抑制i-LTC4的增加。结果表明，LTs可能在PAF诱导的小鼠致死性中起重要作用。
