Mariet F, Brossier P
Unité d'immunoanalyse, Faculté de Pharmacie, Dijon, France.
Res Commun Chem Pathol Pharmacol. 1990 May;68(2):251-62.
The convenient use of solvents as a method to separate free (F) and bound (B) fractions in drug immunoassays which utilize organometallic moieties as labels, has been developed. Two types of drugs labelled with an organometallic complex and denominated metallohaptens (or metallotracers) are studied as model assays: desipramine (tricyclic antidepressant) and phenobarbital (antiepileptic agent). Different organic solvents have potentialities to extract these metallohaptens. To illustrate this solvent procedure we describe antiserum dilution curves and we compare the results with those obtained for the same samples using radiotracers in conjunction with dextran coated charcoal as separation agent. This immunoassay method which associates a metallotracer and B/F solvent separation appears to be fast, cheap, without the step of centrifugation.
已经开发出一种方便的方法，即在利用有机金属部分作为标记的药物免疫分析中，使用溶剂来分离游离（F）和结合（B）部分。研究了两种用有机金属络合物标记并称为金属半抗原（或金属示踪剂）的药物作为模型分析：地昔帕明（三环类抗抑郁药）和苯巴比妥（抗癫痫药）。不同的有机溶剂具有提取这些金属半抗原的潜力。为了说明这种溶剂方法，我们描述了抗血清稀释曲线，并将结果与使用放射性示踪剂结合葡聚糖包被活性炭作为分离剂对相同样品获得的结果进行了比较。这种将金属示踪剂与B/F溶剂分离相结合的免疫分析方法似乎快速、廉价，无需离心步骤。
