Mariet F, Brossier P
Unité d'immunoanalyse, Faculté de Pharmacie, Dijon, France.
Pharm Acta Helv. 1991;66(2):60-4.
In order to develop a new immunoassay procedure based on the use of organometallic labels, antisera are raising against sedative-hypnotic drug: phenobarbital. Immunization was performed in rabbits with parasuccinylamidophenobarbital coupled to bovine-serum-albumin. The evaluation of the specificity and the affinity constants of antisera are studied against different hypnotic or antiepileptic drugs and the metallotracer (phenobarbital labelled with an organometallic fragment derived from cymantrene). The most important result of this study indicates that the organometallic moiety of the metallotracer did not disturb neither the inhibition (% inhibition = 110) nor the affinity constant (Ka = 2.10(8) L/M) of the phenobarbital.
为了开发一种基于有机金属标记物的新型免疫分析方法，正在制备针对镇静催眠药物苯巴比妥的抗血清。用与牛血清白蛋白偶联的对琥珀酰氨基苯巴比妥对兔子进行免疫。针对不同的催眠或抗癫痫药物以及金属示踪剂（用来自 cymantrene 的有机金属片段标记的苯巴比妥）研究抗血清的特异性和亲和常数。该研究的最重要结果表明，金属示踪剂的有机金属部分既不干扰苯巴比妥的抑制作用（抑制率 = 110）也不干扰其亲和常数（Ka = 2.10(8) L/M）。
