Graduate Institute of Chinese Medicine, China Medical University, Taichung 40402, Taiwan.
Evid Based Complement Alternat Med. 2013;2013:262391. doi: 10.1155/2013/262391. Epub 2013 Mar 4.
Urine therapy has been commonly practiced in ancient civilizations including those of India, China, and Greece. The traditional Chinese medicine KWLL, the precipitation of human urine, has been used in China to alleviate the symptoms of asthma for thousands of years. However, the mechanism of action by which KWLL exerts its immunotherapy is unclear. This study attempted to elucidate the pharmacology of KWLL in mice that had been challenged recurrently by Dermatophagoides pteronyssinus (Der p). BALB/c mice were orally administered KWLL (1 g/kg) before an intratracheal (i.t.) challenge of Der p. Allergic airway inflammation and remodeling were provoked by repetitive Der p (50 μ g/mice) challenges six times at 1 wk intervals. Airway hypersensitivity, histological lung characteristics, and the expression profiles of cytokines and various genes were assessed. KWLL reduced Der p-induced airway hyperresponsiveness and inhibited eosinophil infiltration by downregulating the protein expression of IL-5 in bronchoalveolar lavage fluid (BALF). It also inhibited neutrophil recruitment by downregulating IL-17A in BALF. KWLL effectively diminished inflammatory cells, goblet cell hyperplasia, and mRNA expression of IL-6 and IL-17A in the lung. The reduction by KWLL of airway inflammatory and hyperresponsiveness in allergic asthmatic mice was mediated via immunomodulation of IL-5, IL-6, and IL-17A.
尿疗法在古文明中被广泛应用，包括印度、中国和希腊。在中国，传统中药 KWLL（人尿沉淀物）数千年来一直被用于缓解哮喘症状。然而，KWLL 发挥免疫疗法作用的机制尚不清楚。本研究试图阐明 KWLL 在反复接受屋尘螨（Der p）挑战的小鼠中的药理学作用。BALB/c 小鼠在接受 Der p 气管内（i.t.）挑战前口服 KWLL（1g/kg）。通过在 1 周间隔内重复 6 次给予 50 μ g/只 Der p 来引发过敏性气道炎症和重塑。评估气道高反应性、肺组织学特征以及细胞因子和各种基因的表达谱。KWLL 通过下调支气管肺泡灌洗液（BALF）中 IL-5 的蛋白表达，减轻了 Der p 诱导的气道高反应性并抑制了嗜酸性粒细胞浸润。它还通过下调 BALF 中的 IL-17A 抑制中性粒细胞募集。KWLL 有效减少了肺中的炎症细胞、杯状细胞增生以及 IL-6 和 IL-17A 的 mRNA 表达。KWLL 通过调节 IL-5、IL-6 和 IL-17A 减轻了过敏性哮喘小鼠的气道炎症和高反应性。
