School of Chinese Medicine, College of Chinese Medicine, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, Taiwan; Department of Chinese Medicine, China Medical University Hospital, Taichung 40402, Taiwan.
School of Post-Baccalaureate Chinese Medicine, College of Chinese Medicine, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, Taiwan.
J Ethnopharmacol. 2018 Dec 5;227:181-190. doi: 10.1016/j.jep.2018.08.028. Epub 2018 Aug 30.
In Asia, Jin Gui Shen Qi Wan (JGSQW) has been used for hundreds of years to treat asthma.
The traditional Chinese medicine JGSQW is composed of Rehmannia glutinosa, Dioscorea opposita, Cornus officinalis, Poria cocos, Paeonia suffruticosa, Alisma orientalis, Aconitum carmichaelii and Cinnamomum cassia. However, the immunological mechanism underlying the effect of JGSQW treatment on asthma remains unclear. This study examined whether JGSQW has the potential to reduce asthma symptoms in mice with chronic asthma induced by recurrent Dermatophagoides pteronyssinus (Der p) stimulation, as well as its immunoregulatory mechanisms.
The airways of BALB/c mice were stimulated with Der p (i.t.) once per week (50 μL, 1 mg/mL) for 6 consecutive weeks, and the mice were fed JGSQW (1 g/kg) 30 min prior to the Der p stimulation. Three days after the last stimulation, the mice were sacrificed to evaluate the airway remodelling, infiltration of inflammatory cells, lung histological features, and total IgE in the blood. Additionally, after A549 cells were treated with JGSQW, loganin, or paeoniflorin for 30 min, 10 ng/mL IL-1β was added to stimulate the A549 cells to evaluate the effect of the medicine on the ICAM-1 gene expression after IL-1β stimulation.
JGSQW significantly reduced the Der p-induced infiltration of inflammatory cells into airways and decreased the total IgE and Der p-specific IgG1 in serum. Collagen assays and histopathological examinations showed that JGSQW reduced lung airway remodelling. Additionally, an electrophoretic mobility shift assay and immunohistochemical staining verified that JGSQW inhibited the NF-kB expression in airway epithelial cell nuclei. JGSQW, loganin, and paeoniflorin inhibited the ICAM-1 gene expression caused by the IL-1β stimulation of A549 cells, and loganin and paeoniflorin had the maximum inhibitory effect when mixed according to the combination of doses in JGSQW.
These results indicated that in the chronic asthma mouse model, JGSQW inhibits the infiltration of inflammatory cells into the airways and airway remodelling and exhibits specific immunoregulatory effects. JGSQW with certain doses of loganin and paeoniflorin inhibited ICAM-1 gene expression in epithelial cells.
在亚洲，金匮肾气丸（JGSQW）已被用于治疗哮喘数百年。
中药 JGSQW 由熟地黄、山药、山茱萸、茯苓、牡丹皮、泽泻、制川乌和肉桂组成。然而，JGSQW 治疗哮喘的免疫机制尚不清楚。本研究探讨了 JGSQW 是否具有减轻由反复屋尘螨（Der p）刺激引起的慢性哮喘小鼠哮喘症状的潜力，以及其免疫调节机制。
BALB/c 小鼠的气道每周通过 i.t. 刺激一次（50 μL，1 mg/mL），连续 6 周，在 Der p 刺激前 30 min 给予 JGSQW（1 g/kg）。最后一次刺激后 3 天，处死小鼠评估气道重塑、炎症细胞浸润、肺组织学特征和血液中的总 IgE。此外，在 JGSQW、马钱苷或芍药苷处理 A549 细胞 30 min 后，加入 10ng/mL IL-1β刺激 A549 细胞，评估药物对 IL-1β刺激后 ICAM-1 基因表达的影响。
JGSQW 显著减少了 Der p 诱导的炎症细胞浸润到气道，并降低了血清中的总 IgE 和 Der p 特异性 IgG1。胶原蛋白测定和组织病理学检查显示 JGSQW 减少了肺气道重塑。此外，电泳迁移率变动分析和免疫组织化学染色证实 JGSQW 抑制了气道上皮细胞核中 NF-kB 的表达。JGSQW、马钱苷和芍药苷抑制了 IL-1β刺激 A549 细胞引起的 ICAM-1 基因表达，并且当根据 JGSQW 中的剂量组合混合时，马钱苷和芍药苷具有最大的抑制作用。
这些结果表明，在慢性哮喘小鼠模型中，JGSQW 抑制炎症细胞浸润到气道和气道重塑，并表现出特定的免疫调节作用。JGSQW 与一定剂量的马钱苷和芍药苷抑制上皮细胞中 ICAM-1 基因的表达。
