Wasinger Valerie C, Zeng Ming, Yau Yunki
Bioanalytical Mass Spectrometry Facility, Mark Wainwright Analytical Centre, The University of New South Wales, Sydney, NSW 2052, Australia.
Int J Proteomics. 2013;2013:180605. doi: 10.1155/2013/180605. Epub 2013 Mar 6.
The accurate quantitation of proteins and peptides in complex biological systems is one of the most challenging areas of proteomics. Mass spectrometry-based approaches have forged significant in-roads allowing accurate and sensitive quantitation and the ability to multiplex vastly complex samples through the application of robust bioinformatic tools. These relative and absolute quantitative measures using label-free, tags, or stable isotope labelling have their own strengths and limitations. The continuous development of these methods is vital for increasing reproducibility in the rapidly expanding application of quantitative proteomics in biomarker discovery and validation. This paper provides a critical overview of the primary mass spectrometry-based quantitative approaches and the current status of quantitative proteomics in biomedical research.
在复杂生物系统中对蛋白质和肽进行准确定量，是蛋白质组学中最具挑战性的领域之一。基于质谱的方法已经取得了重大进展，能够实现准确、灵敏的定量分析，并且通过应用强大的生物信息学工具，具备对极其复杂的样本进行多重分析的能力。这些使用无标记、标签或稳定同位素标记的相对和绝对定量方法都有各自的优缺点。这些方法的持续发展对于在生物标志物发现和验证中快速扩展的定量蛋白质组学应用中提高重现性至关重要。本文对基于质谱的主要定量方法以及生物医学研究中定量蛋白质组学的现状进行了批判性综述。
