Food Safety Division, ANFACO-CECOPESCA, Campus Univ. 16, 36310 Vigo PO, Spain.
J Agric Food Chem. 2013 May 1;61(17):4195-203. doi: 10.1021/jf3050186. Epub 2013 Apr 18.
The toxic effects of the organotin compounds (OTCs) monobutyltin (MBT), dibutyltin (DBT), and tributyltin (TBT) were evaluated in vitro in a neuroblastoma human cell line. Mechanisms of cell death, apoptosis versus necrosis, were studied by using several markers: inhibition of cell viability and proliferation, F-actin, and mitochondrial membrane potential changes as well as reactive oxygen species (ROS) production and DNA fragmentation. The most toxic effects were detected with DBT and TBT even at very low concentrations (0.1-1 μM). In contrast, MBT induced lighter cytotoxic changes at the higher doses tested. None of the studied compounds stimulated propidium iodide uptake, although the most toxic chemical, TBT, caused lactate dehydrogenase release at the higher concentrations tested. These findings suggest that in neuroblastoma, OTC-induced cytotoxicity involves different pathways depending on the compound, concentration, and incubation time. A screening method for DBT and TBT quantification based on cell viability loss was developed, allowing a fast detection alternative to complex methodology.
研究了有机锡化合物（OTCs）单丁基锡（MBT）、二丁基锡（DBT）和三丁基锡（TBT）在人神经母细胞瘤细胞系中的体外毒性作用。通过使用几种标记物研究了细胞死亡的机制，即细胞凋亡与坏死：抑制细胞活力和增殖、F-肌动蛋白和线粒体膜电位变化以及活性氧（ROS）产生和 DNA 片段化。即使在非常低的浓度（0.1-1 μM）下，DBT 和 TBT 也检测到了最毒的影响。相比之下，在测试的较高剂量下，MBT 诱导的细胞毒性变化较轻。研究的化合物均未刺激碘化丙啶摄取，尽管毒性最强的化学物质 TBT 在测试的较高浓度下导致乳酸脱氢酶释放。这些发现表明，在神经母细胞瘤中，OTC 诱导的细胞毒性取决于化合物、浓度和孵育时间，涉及不同的途径。建立了一种基于细胞活力丧失的 DBT 和 TBT 定量筛选方法，为复杂方法提供了一种快速检测替代方法。
