Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, PL 90-237 Łódź, Poland.
J Hazard Mater. 2013 Feb 15;246-247:277-82. doi: 10.1016/j.jhazmat.2012.12.034. Epub 2012 Dec 28.
Organotin compounds are ubiquitous in environment. However, biodegradation of tributyltin (TBT) and dibutyltin (DBT) to non toxic metabolites by fungi has been seldom observed. In this study we constructed a fungal co-culture with an efficient ability of TBT and its metabolites removal. The microscopic fungus strain Cunninghamella elegans degraded TBT via hydroxybutyldibutyltin (OHBuDBT) to its metabolites: DBT and monobutyltin (MBT), which were then transformed by Cochliobolus lunatus. The sequential biodegradation resulted in a 10-fold decrease in samples toxicity to Artemia franciscana larvae. With an initial TBT concentration of 5 mg l(-1), the co-culture of both fungi almost completely eliminated butyltins during 12 days of incubation in synthetic medium. To our knowledge, this is the first report that the mixed fungal co-culture could efficiently degrade TBT. This process was associated with glucose utilization, and a cometabolic nature of butyltins removal by selected strains has been suggested.
有机锡化合物在环境中无处不在。然而，真菌将三丁基锡（TBT）和二丁基锡（DBT）生物降解为无毒代谢物的情况很少见。在这项研究中，我们构建了一种真菌共培养体，具有高效去除 TBT 及其代谢物的能力。微小真菌菌株 Cunninghamella elegans 通过羟基丁基二丁基锡（OHBuDBT）将 TBT 降解为其代谢物：DBT 和单丁基锡（MBT），然后由 Cochliobolus lunatus 转化。连续的生物降解导致样品对弗朗西斯科湾卤虫幼虫的毒性降低了 10 倍。在初始 TBT 浓度为 5 mg l(-1)的情况下，两种真菌的共培养体在合成培养基中孵育 12 天几乎完全消除了丁基锡。据我们所知，这是第一个报道混合真菌共培养体可以有效降解 TBT 的报告。这个过程与葡萄糖的利用有关，并且建议选择的菌株通过共代谢作用去除丁基锡。
