Iida H, Kast A
Department of Experimental Pathology, Nippon Boehringer Ingelheim Co., Ltd., Kawanishi Pharma Research Institute, Hyogo, Japan.
Z Versuchstierkd. 1990;33(2):100-6.
The study compared the fertility of nulliparous Jcl:SD rats, young mature (12-week-old) versus middle aged (47-week-old) or old animals (64-week-old), both groups near the termination of their reproductive capability. Trypan blue (TB) was given subcutaneously at 0 (control), 10 or 50 mg/kg on days 7 through 9 of gestation to increase the malformation rate. However, no clear teratogenic action of TB was observed. The dose of 10 mg/kg TB was well tolerated, while 50 mg/kg was of severe maternal toxicity and 14 of 53 females died. In comparison with the young control group, the average per litter from middle aged or old dams was significantly decreased for implantation sites, live fetuses and fetal weight, while the percentages of pre-implantation loss and dead implants were extremely high. No increase of the malformation rate occurred with maternal aging. Histologically, middle aged and old rats exhibited age-related lesions of the pituitary, adrenals and reproductive organs with pituitary adenomas, cystic endometrium and sclerotic atrophy of uterine glands being significantly increased in non-pregnant, old rats.
该研究比较了未生育的Jcl:SD大鼠的生育能力，将年轻成熟（12周龄）与中年（47周龄）或老年动物（64周龄）进行对比，这两组动物均接近其生殖能力终止期。在妊娠第7至9天，分别以0（对照）、10或50mg/kg的剂量皮下注射台盼蓝（TB）以提高畸形率。然而，未观察到TB有明显的致畸作用。10mg/kg TB的剂量耐受性良好，而50mg/kg则有严重的母体毒性，53只雌性中有14只死亡。与年轻对照组相比，中年或老年母鼠每窝的着床部位、活胎数量和胎儿体重均显著减少，而着床前损失和植入后死亡的百分比极高。母体衰老并未导致畸形率增加。组织学上，中年和老年大鼠表现出与年龄相关的垂体、肾上腺和生殖器官病变，非妊娠老年大鼠的垂体腺瘤、囊性子宫内膜和子宫腺硬化性萎缩显著增加。
