Departament de Química Analítica, Facultat de Química, Universitat de Barcelona, Barcelona, Spain.
Anal Bioanal Chem. 2013 May;405(14):4777-86. doi: 10.1007/s00216-013-6896-z. Epub 2013 Mar 28.
An analytical method for determination and confirmation of nine coccidiostatics in eggs is reported. Ethyl acetate is used as extraction solvent, with satisfactory results, and simple automated clean-up is based on gel-permeation chromatography (GPC) . The target compounds are then analysed by liquid chromatography-electrospray ionization-tandem mass spectrometry. The method was validated in-house in accordance with Commission Decision 2002/657/EC. Trueness and precision were determined at four concentrations, and the mean errors obtained were <10 %, with relative standard deviations ranging from 3 to 18 %. For three non-authorized coccidiostatics (clopidol, ethopabate, and ronizadole), decision limit and detection capability were in the ranges 0.12-0.16 and 0.18-0.23 μg kg(-1), respectively. The results obtained prove the suitability of this new analytical method for routine monitoring of these substances in eggs.
本文报道了一种用于鸡蛋中 9 种抗球虫药的检测和确证的分析方法。采用乙酸乙酯作为提取溶剂，结果令人满意，且简单的自动净化基于凝胶渗透色谱（GPC）。然后，通过液相色谱-电喷雾串联质谱法对目标化合物进行分析。该方法按照委员会第 2002/657/EC 号决定在内部进行了验证。在 4 个浓度下进行了准确度和精密度的测定，得到的平均误差小于 10%，相对标准偏差范围为 3-18%。对于 3 种未经授权的抗球虫药（氯羟吡啶、乙氧酰胺苯甲酯和罗硝唑），其决策限和检测能力分别为 0.12-0.16μg/kg 和 0.18-0.23μg/kg。所得结果证明了这种新的分析方法适合用于鸡蛋中这些物质的常规监测。
