Department of Physical Chemistry, Medical University of Lublin, ul. Chodźki 4a, 20-093 Lublin, Poland.
Molecules. 2020 Dec 18;25(24):6011. doi: 10.3390/molecules25246011.
Coccidiostats are drugs used against coccidiosis, a common disease among breeding animals. Their widespread application leads to the appearance of their residues in food, which is potentially harmful for human health and life. The European Union has established limits of concentrations of these drugs in premixtures and food. Nowadays, there are many methods for monitoring coccidiostats' presence in market products, but their frequent weakness is sample preparation. Solvent Front Position Extraction is a planar chromatography-based sample preparation method that allows for effective assay of samples with coccidiostats when coupled with LC-MS/MS. The purpose of this research was to find common conditions for the effective isolation of eight coccidiostats from biological sample components with both lower and higher retention than the substances of interest. The acquired results were used for effective isolation of monensin and salinomycin from the premixture samples and allowed for their quantitative determination. The application of a semi-automatic device for the development of chromatograms positively impacted the results, confirming the effectiveness of the method for determining coccidiostats in biological samples.
球虫抑制剂是用于对抗球虫病的药物，球虫病是一种在养殖动物中常见的疾病。它们的广泛应用导致其残留出现在食物中，这对人类健康和生命构成潜在威胁。欧盟已经制定了这些药物在预混料和食品中的浓度限量。如今，有许多监测市场产品中球虫抑制剂存在的方法，但它们经常存在的一个弱点是样品制备。前沿位置萃取是一种基于平面色谱的样品制备方法，当与 LC-MS/MS 结合使用时，可有效检测含有球虫抑制剂的样品。本研究的目的是找到有效分离八种球虫抑制剂的通用条件，这些抑制剂的保留时间比感兴趣的物质低或高。所获得的结果用于从预混料样品中有效分离莫能菌素和盐霉素，并允许对其进行定量测定。半自动化设备用于开发色谱图对结果产生了积极影响，证实了该方法在确定生物样品中球虫抑制剂方面的有效性。
