Dhaliwal Ravinder S, Kitchell Barbara E, Ehrhart Ej, Valli Victor E, Dervisis Nikolaos G
Center for Comparative Oncology, Michigan State University, East Lansing, MI, USA.
J Am Anim Hosp Assoc. 2013 May-Jun;49(3):175-84. doi: 10.5326/JAAHA-MS-5843. Epub 2013 Mar 27.
To characterize the expression of P-glycoprotein (Pgp) and p53 in different histologic grades of canine multicentric lymphosarcoma (LSA), 31 cases of LSA without prior treatment were studied. The expression levels of the Pgp and p53 proteins were evaluated for their clinicopathologic significance among standard histologic evaluation. Immunohistochemistry (IHC) was performed on formalin-fixed, paraffin-embedded archival samples of 31 previously untreated LSA cases to detect the expression of Pgp and p53. All dogs were subsequently treated with a combination chemotherapy protocol. Remission and survival durations were evaluated for correlation with histologic grade and presence of drug resistance markers. Of the 31 cases, 24 (80%) and 7 (22%) were positive for Pgp and p53, respectively. Overall, the median survival and duration of remission in the study was 246 days and 137 days, respectively. The National Cancer Institute working formulation histologic grade was not associated with either survival or duration of first remission (DOR). The Pgp protein expression and DOR and survival was not statistically significant. Expression of p53 was statistically correlated with survival.
为了表征P-糖蛋白（Pgp）和p53在不同组织学分级的犬多中心淋巴瘤（LSA）中的表达情况，对31例未经治疗的LSA病例进行了研究。在标准组织学评估中，评估Pgp和p53蛋白的表达水平及其临床病理意义。对31例先前未经治疗的LSA病例的福尔马林固定、石蜡包埋存档样本进行免疫组织化学（IHC）检测，以检测Pgp和p53的表达。所有犬随后均接受联合化疗方案治疗。评估缓解期和生存期与组织学分级及耐药标志物存在情况的相关性。在31例病例中，Pgp和p53阳性分别为24例（80%）和7例（22%）。总体而言，该研究中的中位生存期和缓解期分别为246天和137天。美国国立癌症研究所工作分类组织学分级与生存期或首次缓解期（DOR）均无关。Pgp蛋白表达与DOR和生存期无统计学意义。p53表达与生存期具有统计学相关性。
