Department of Chemical Engineering, Kwangwoon University, Seoul, 139-701, Republic of Korea.
Appl Biochem Biotechnol. 2013 Nov;171(5):1118-27. doi: 10.1007/s12010-013-0189-z. Epub 2013 Mar 29.
A semi-pilot continuous process (SPCP) for enzymatic biodiesel synthesis utilizing near-critical carbon dioxide (NcCO₂) as the reaction medium was developed with the aim of reducing the reaction time and alleviating the catalyst inhibition by methanol. Biodiesel synthesis was evaluated in both lab-scale and semi-pilot scale reactors (batch and continuous reactors). In a SPCP, the highest conversion (∼99.9 %) in four and a half hours was observed when three-step substrate (methanol) addition (molar ratio [oil/methanol] = 1:1.3) was used and the reaction mixture containing enzyme (Lipozyme TL IM, 20 wt.% of oil) was continuously mixed (agitation speed = 300 rpm) at 30 °C and 100 bar in a CO₂ environment. The biodiesel produced from canola oil conformed to the fuel standard (EU) even without additional downstream processing, other than glycerol separation and drying.
利用近临界二氧化碳（NcCO₂）作为反应介质，开发了一种用于酶法生物柴油合成的半连续过程（SPCP），旨在缩短反应时间并减轻甲醇对催化剂的抑制作用。在实验室规模和半工业规模（分批和连续）反应器中评估了生物柴油的合成。在 SPCP 中，当使用三步底物（甲醇）添加（摩尔比[油/甲醇]=1:1.3）且反应混合物中含有酶（Lipozyme TL IM，油的 20wt.%）在 30°C 和 100 巴的 CO₂环境下连续混合（搅拌速度=300rpm）时，观察到四个半小时内的最高转化率（约 99.9%）。从菜籽油生产的生物柴油无需额外的下游加工，除了甘油分离和干燥，即可符合燃料标准（EU）。
