Peng Zhao, Wu Ke, Tong Qiang, Wang Guo-bin
Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Zhonghua Wei Chang Wai Ke Za Zhi. 2013 Mar;16(3):256-9.
To identify the expression of DOG-1 in gastrointestinal stromal tumors (GIST) and to explore its potential association with clinicopathological features of GIST.
Two tissue microarrays (TMA) were used for the study. Each TMA contained 80 tissue samples of GIST from 80 different patients, with each tumor represented by one core, and paraffin-embedded sections of 40 samples from normal gastrointestinal tissue were used as control. Immunohistochemistry staining (SABC method) was performed on TMA and paraffin-embedded sections to detected the expression of c-Kit (CD117) and DOG-1.
Immunohistochemistry showed that in 80 GIST patients, 76 cases (95.0%) were DOG-1 positive and 67 cases (83.8%) were CD117 positive. The positive rate of DOG-1 was higher than that of CD117 (P<0.05). In 13 GIST samples of negative CD117, the positive rate of DOG-1 was 100%. Expressions of both DOG-1 and CD117 were negative in all the 40 samples of normal gastrointestinal tissue. The positive expression of DOG-1 and CD117 was not significantly different in spindle cell type (96.0% vs. 96.0%, P>0.05) and in mixed cell type (91.7% vs. 75.0%, P>0.05). While in the epithelioid cell type, the DOG-1 expression was higher than CD117 expression (94.1% vs. 52.9%, P<0.05). The positive expression of DOG-1 and CD117 was not associated with age, gender, location and risk stratification of the tumors (all P>0.05).
DOG-1 expression is up-regulated in gastrointestinal stromal tumors, especially in epithelioid cell GIST, and may be used as a new marker in the diagnosis of GIST.
检测DOG-1在胃肠道间质瘤（GIST）中的表达情况，并探讨其与GIST临床病理特征的潜在关联。
采用两个组织芯片（TMA）进行研究。每个TMA包含来自80例不同患者的80个GIST组织样本，每个肿瘤用一个组织芯代表，同时选取40例正常胃肠道组织的石蜡包埋切片作为对照。对TMA和石蜡包埋切片进行免疫组织化学染色（SABC法），以检测c-Kit（CD117）和DOG-1的表达。
免疫组织化学显示，80例GIST患者中，76例（95.0%）DOG-1阳性，67例（83.8%）CD117阳性。DOG-1阳性率高于CD117（P<0.05）。在13例CD117阴性的GIST样本中，DOG-1阳性率为100%。40例正常胃肠道组织样本中DOG-1和CD117表达均为阴性。DOG-1和CD117在梭形细胞型（96.0%对96.0%，P>0.05）和混合细胞型（91.7%对75.0%，P>0.05）中的阳性表达无显著差异。而在上皮样细胞型中，DOG-1表达高于CD117表达（94.1%对52.9%，P<0.05）。DOG-1和CD117的阳性表达与肿瘤的年龄、性别、部位及危险度分级均无关（均P>0.05）。
DOG-1在胃肠道间质瘤中表达上调，尤其是在上皮样细胞型GIST中，可作为GIST诊断的新标志物。
