1Laboratory of Gynecological Oncology, The Hospital on Integration of Chinese and Western Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, PR China.
Reprod Sci. 2013 Oct;20(10):1215-23. doi: 10.1177/1933719113483008. Epub 2013 Mar 27.
The objective of this study is to investigate the effect of Wenshen Xiaozheng Tang (WXT) on the development of endometriosis in a rat model. Sprague-Dawley rats in which endometriotic implants were induced were divided randomly into 3 groups. The rats in the low-dose and high-dose WXT groups were administered WXT 8.57 and 17.14 g/kg/d, respectively. The rats in the control groups received an equal volume of dissolvent, as did the sham-operated rats. After treatment for 4 weeks, WXT significantly decreased the mean lesion size as well as the peritoneal fluid and serum levels of tumor necrosis factor α and interleukin 1β. Cyclooxygenase-2, matrix metalloproteinase 9, plasminogen activator inhibitor 1, and intercellular adhesion molecule 1 messenger RNA (mRNA) levels were downregulated, and the mRNA expression of tissue inhibitor of metalloproteinase 1 was upregulated in the endometriotic lesions of WXT versus control group. Our data suggested that WXT may suppress the development of endometriosis by inhibiting the production of proinflammatory cytokines and regulating the expression of invasion-related genes in the endometriotic lesions.
本研究旨在探讨温肾消癥汤（WXT）对子宫内膜异位症大鼠模型发病的影响。将诱导子宫内膜异位种植的 Sprague-Dawley 大鼠随机分为 3 组，WXT 低、高剂量组分别给予 WXT8.57、17.14g/kg/d，对照组给予等量溶剂，假手术组大鼠给予同体积溶剂。治疗 4 周后，WXT 显著降低了平均病灶大小以及腹腔液和血清中肿瘤坏死因子-α 和白细胞介素 1β 的水平。WXT 组异位病灶中环氧合酶-2、基质金属蛋白酶 9、纤溶酶原激活物抑制剂 1 和细胞间黏附分子 1 的信使 RNA（mRNA）水平下调，组织金属蛋白酶抑制剂 1 的 mRNA 表达上调。我们的数据表明，WXT 可能通过抑制促炎细胞因子的产生和调节异位病灶中侵袭相关基因的表达来抑制子宫内膜异位症的发展。
