一种新型 HIV-1 RNA 定量 PCR 检测方法。
A novel PCR assay for quantification of HIV-1 RNA.
机构信息
Department of Medicine, Hopkins University School of Medicine, Baltimore, Maryland, USA.
出版信息
J Virol. 2013 Jun;87(11):6521-5. doi: 10.1128/JVI.00006-13. Epub 2013 Mar 27.
Abstract
Current assays for quantification of HIV-1 virions rely on real-time reverse transcriptase (RT)-PCR detection of conserved regions of HIV-1 RNA and can be limited by detection of contaminating viral or plasmid DNA. We developed a novel RT-PCR assay using a reverse primer that hybridizes with the poly(A) tail of HIV-1 mRNAs, anchored by conserved viral nucleotides at the most distal region of the transcript. This assay can detect and quantify HIV-1 RNA with high specificity and sensitivity.
摘要
目前用于定量检测 HIV-1 病毒颗粒的方法依赖于实时逆转录酶（RT）-PCR 检测 HIV-1 RNA 的保守区域，并且可能受到检测污染的病毒或质粒 DNA 的限制。我们开发了一种新型 RT-PCR 检测方法，使用与 HIV-1 mRNA 的 poly(A) 尾巴杂交的反向引物，该引物由转录本最远端的保守病毒核苷酸固定。该检测方法具有高度特异性和灵敏度，可以检测和定量 HIV-1 RNA。
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