Guérin J L, Favre G, Raynal G, Soula G
Département de Biologie Clinique, Centre Claudius-Régaud, Toulouse.
Ann Biol Clin (Paris). 1990;48(3):167-70.
The authors have adapted a turbidimetric assay on centrifugal Cobas Fara analyser for cerebrospinal fluid proteins determinations. The later method is proposed by SFBC Protein Analysis Committee using benzethonium chloride as monoreagent. This adaptation is more sensitive than manual method. The linearity range is greater (0.08-1.85 g/l instead of 0.2-1.2 g/l). Sample assay and time of analysis were reduced by 90 p. cent.
作者们在离心式Cobas Fara分析仪上采用了比浊法来测定脑脊液蛋白。后一种方法是由SFBC蛋白质分析委员会提出的,使用苄索氯铵作为单一试剂。这种改进后的方法比手工方法更灵敏。线性范围更大(0.08 - 1.85 g/l,而不是0.2 - 1.2 g/l)。样本测定和分析时间减少了90%。