Flachaire E, Later R, Caudie C, Quincy C
Ann Biol Clin (Paris). 1983;41(1):39-44.
We have adapted Iwata and Nishikaze's benzethonium chloride turbidimetric method for the assay of CSF proteins to the techniques of continuous flow and the centrifuge analyser. The proposed technique presents several advantages: benzethonium chloride forms a stable precipitate with the proteins with a slow kinetic of formation, enabling adaptation to the centrifuge analyser; the response obtained is identical for albumin and immunoglobulin G; this very sensitive technique allows the use of micro-samples. Studies of repeatability and the correlations made with other techniques give very satisfactory results.
我们已将岩田和西风的用于脑脊液蛋白质测定的苄索氯铵比浊法,适配于连续流动技术和离心分析仪技术。所提出的技术具有几个优点:苄索氯铵与蛋白质形成稳定沉淀,形成动力学缓慢,能够适配离心分析仪;对白蛋白和免疫球蛋白G获得的响应相同;这种非常灵敏的技术允许使用微量样本。重复性研究以及与其他技术的相关性研究给出了非常令人满意的结果。