College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
Gene. 2013 Jun 15;522(2):206-13. doi: 10.1016/j.gene.2013.03.030. Epub 2013 Mar 26.
The cysteine and glycine-rich protein 1 and 2 genes (CSRP1 and CSRP2) are an effective growth factor in promoting skeletal muscle growth in vitro and vivo. However, in cattle, the information on the CSRP1 and CSRP2 genes is very limited. The aim of this study was to examine the association of the CSRP1 and CSRP2 variants with growth and carcass traits in cattle breeds. Three single nucleotide variants (SNVs) were identified within the bovine CSRP1 gene, whereas CSRP2 gene has not detected any SNVs, using DNA pooled sequencing, PCR-RFLP, and forced PCR-RFLP methods. These SNVs include g. 801T>C (Intron 2), g. 46T>C (Exon 3) and g. 99C>G (Intron 3). Besides, we also investigated haplotype frequencies and linkage disequilibrium (LD) coefficients for three SNVs in all study populations. LD and haplotype structure of CSRP1 were different between breeds. The result of haplotype analysis demonstrated eight haplotype present in QC (Qinchuan) and one haplotype in CH (Chinese Holstein). Only haplotype 1 (TTC), shared by all two populations, comprised 10.74% and 100.00%, of all haplotypes observed in QC and CH, respectively. Haplotype 5 (CTC) had the highest haplotype frequencies in QC (30.98%) and haplotype 1 had the highest haplotype frequencies in CH (100.00%). The statistical analyses indicated that one single SNV and 19 combined haplotypes were significantly or highly significantly associated with growth and carcass traits in the QC cattle population (P<0.05 or P<0.01). Quantitative real-time PCR (qRT-PCR) analyses showed that the bovine CSRP1 and CSRP2 genes were widely expressed in many tissues. The results of this study suggest that the CSRP1 gene possibly is a strong candidate gene that affects growth and carcass traits in the Chinese beef cattle breeding.
富含半胱氨酸和甘氨酸蛋白 1 和 2 基因（CSRP1 和 CSRP2）是一种有效的生长因子，可促进体内外骨骼肌的生长。然而，在牛中，CSRP1 和 CSRP2 基因的信息非常有限。本研究旨在探讨 CSRP1 和 CSRP2 变异与牛品种生长和胴体性状的关系。使用 DNA 池测序、PCR-RFLP 和强制 PCR-RFLP 方法，在牛 CSRP1 基因中鉴定出 3 个单核苷酸变异（SNV），而 CSRP2 基因未检测到任何 SNV。这些 SNV 包括 g.801T>C（内含子 2）、g.46T>C（外显子 3）和 g.99C>G（内含子 3）。此外，我们还研究了所有研究群体中三个 SNV 的单倍型频率和连锁不平衡（LD）系数。CSRP1 的 LD 和单倍型结构在品种之间存在差异。单倍型分析结果表明，QC（秦川）存在 8 种单倍型，CH（中国荷斯坦）存在 1 种单倍型。只有两种群体共有的单倍型 1（TTC）分别占 QC 和 CH 观察到的所有单倍型的 10.74%和 100.00%。QC 中单倍型 5（CTC）的单倍型频率最高（30.98%），而 CH 中单倍型 1 的单倍型频率最高（100.00%）。统计分析表明，一个单 SNV 和 19 个组合单倍型与 QC 牛群体的生长和胴体性状显著或高度显著相关（P<0.05 或 P<0.01）。实时定量 PCR（qRT-PCR）分析表明，牛 CSRP1 和 CSRP2 基因在许多组织中广泛表达。本研究结果表明，CSRP1 基因可能是影响中国肉牛生长和胴体性状的一个强候选基因。
