a College of Veterinary Medicine , Northwest Agriculture and Forestry University , Yangling , Shaanxi , China.
b Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology , Northwest Agriculture and Forestry University , Yangling , Shaanxi , China.
Anim Biotechnol. 2018;29(4):259-268. doi: 10.1080/10495398.2017.1377084. Epub 2017 Nov 2.
TCAP, TNNI1, and FHL1 regulate muscle growth and development. In this study, four single nucleotide variants (SNVs) were discovered in almost all of the exon and intron regions of the TCAP, TNNI1, and FHL1 genes using DNA pooled sequencing, polymerase chain reaction (PCR)-RFLP, and forced-PCR-RFLP methods in 576 cattle. Four SNVs were significantly associated with the growth performance and carcass quality traits of the cattle. In addition, the haplotype, haplotype frequency, and linkage disequilibrium coefficient of three sequence variants were also evaluated in the cattle population. Haplotype analysis demonstrated that eight haplotypes were present in the Qinchuan cattle population and no haplotypes were present in the Chinese Holstein population; haplotype 1 had the highest frequency in the Qinchuan (42.7%) population. Statistical analyses of 12 combined genotypes indicated that some were significantly associated with the growth performance and carcass quality traits of the Qinchuan cattle population. Moreover, the quantitative real-time polymerase chain reaction results demonstrated that the bovine TCAP, TNNI1, and FHL1 genes were exclusively expressed in muscle tissue. These data support the high potentials of the TCAP, TNNI1, and FHL1 as marker genes to improve the growth performance and carcass quality traits of Qinchuan cattle or other animals selection programs.
TCAP、TNNI1 和 FHL1 调节肌肉生长和发育。在这项研究中，使用 DNA 池测序、聚合酶链反应（PCR）-RFLP 和强制 PCR-RFLP 方法，在 576 头牛中发现了 TCAP、TNNI1 和 FHL1 基因的几乎所有外显子和内含子区域的四个单核苷酸变异（SNV）。四个 SNV 与牛的生长性能和胴体质量性状显著相关。此外，还评估了三个序列变异在牛群体中的单倍型、单倍型频率和连锁不平衡系数。单倍型分析表明，秦川牛群体中存在 8 种单倍型，而中国荷斯坦牛群体中不存在单倍型；秦川牛群体中单倍型 1 的频率最高（42.7%）。对 12 种组合基因型的统计分析表明，一些基因型与秦川牛群体的生长性能和胴体质量性状显著相关。此外，定量实时聚合酶链反应结果表明，牛 TCAP、TNNI1 和 FHL1 基因仅在肌肉组织中表达。这些数据支持 TCAP、TNNI1 和 FHL1 作为标记基因的高潜力，以提高秦川牛或其他动物选择计划的生长性能和胴体质量性状。
