一种测定鞘磷脂酶活性的方法。
A method for the determination of sphingomyelinase activity.
作者信息
De Rooij R E, Liem K O, Brouwer-Schipper J W, Husmann G G, Hooghwinkel G J
出版信息
Clin Chim Acta. 1975 Feb 22;59(1):71-9. doi: 10.1016/0009-8981(75)90220-x.
PMID:235385
Abstract
A method is presented for the determination of the activity of sphingomyelinase. It is based on the determination of sphingomyelin before and after enzymatic conversion with the aid of a quantitative paper chromatographic analysis of phospholipids. This method is used for the determination of sphingomyelinase activity in various tissues such as those of the human brain and liver and fibroblast cultures. Normal values are presented.
摘要
本文介绍了一种测定鞘磷脂酶活性的方法。该方法基于借助磷脂的定量纸色谱分析来测定酶促转化前后的鞘磷脂。此方法用于测定各种组织（如人脑、肝脏组织和成纤维细胞培养物）中的鞘磷脂酶活性，并给出了正常值。
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引用本文的文献
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Studies on sphingomyelinase activity in cultured cells and leucocytes.培养细胞和白细胞中鞘磷脂酶活性的研究。
J Inherit Metab Dis. 1978;1(1):29-33. doi: 10.1007/BF01805712.
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Physicochemical characteristics of the glycosaminoglycan-lysosomal enzyme interaction in vitro. A model of control of leucocytic lysosomal activity.体外糖胺聚糖 - 溶酶体酶相互作用的物理化学特性。白细胞溶酶体活性控制模型。
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