Brandão Rosana Pinho, Boehs Guisla, Silva Patrícia Mirella da
Rev Bras Parasitol Vet. 2013 Jan-Mar;22(1):84-91. doi: 10.1590/s1984-29612013005000007. Epub 2013 Mar 19.
This study investigated the health of natural stocks of the oyster Crassostrea rhizophorae on the southern coast of Bahia in northeastern Brazil, during summer and winter 2010, at three localities (sampling points) in the estuaries of the Maraú (Camamu Bay) and Graciosa rivers. A total of 180 oysters (30/sampling point/season) were examined macroscopically for the presence of pathogens and anatomical changes. The specimens were subsequently fixed in Davidson solution, processed for paraffin embedding, sectioned and stained with Harris' hematoxylin and eosin. Histological analysis revealed the presence of Rickettsia-like organisms (RLOs), Ancistrocoma, Trichodina, Sphenophrya, Nematopsis, Urastoma, Bucephalus in the sporocyst phase, a nonspecific metacercaria, and a metacestode of genus Tylocephalum. The prevalence of infection was low except for parasitism by Nematopsis sp. which also caused histopathological changes. The presence of Bucephalus sp. caused parasitic castration. These two pathogens significantly affect the health of C. rhizophorae.
本研究调查了2010年夏季和冬季巴西东北部巴伊亚州南部海岸马劳（卡马穆湾）河和格拉西奥萨河河口三个地点（采样点）的天然根枝牡蛎种群的健康状况。总共检查了180只牡蛎（每个采样点/季节30只），肉眼观察病原体的存在和解剖学变化。随后将标本固定在戴维森溶液中，进行石蜡包埋、切片，并用哈里斯苏木精和伊红染色。组织学分析显示存在立克次氏体样生物（RLOs）、锚首虫、车轮虫、蝶须虫、类线虫、尾口虫、孢子囊期的布氏吸虫、非特异性囊蚴以及泰勒头属的绦虫蚴。除类线虫属寄生外，感染率较低，类线虫属寄生也引起了组织病理学变化。布氏吸虫属的存在导致了寄生性阉割。这两种病原体显著影响了根枝牡蛎的健康。
