Department of Biochemistry and Molecular Biology, Poznań University of Medical Sciences, 6 Święcickiego Street, 60781 Poznań, Poland.
Biomed Pharmacother. 2013 May;67(4):299-303. doi: 10.1016/j.biopha.2012.11.003. Epub 2012 Nov 20.
Leukemia and lymphoma cells are positive for estrogen receptors and contain endogenous estrogens and estrogen metabolites. Therefore, we studied the levels of 17 beta-hydroxysteroid-dehydrogenase type 1 (HSD17B1) transcript and protein and the production of 17 beta-estradiol (E2) from estrone (E1) in human Jurkat acute T cell leukemia, Hut-78 and Raji B lymphoma cells. We found the presence of HSD17B1 transcripts and proteins in these cells. Moreover, we demonstrated that Jurkat, Hut-78, and Raji B cells are able to produce E2 from E1. Our results indicate that Jurkat acute T cell leukemia, Hut-78 and Raji B lymphoma cells are able to convert weak estrogen E1 to the more potent E2 in vitro.
白血病和淋巴瘤细胞表达雌激素受体，且含有内源性雌激素和雌激素代谢物。因此，我们研究了人 Jurkat 急性 T 细胞白血病、Hut-78 和 Raji B 淋巴瘤细胞中 17β-羟类固醇脱氢酶 1 型（HSD17B1）转录本和蛋白的水平，以及从雌酮（E1）生成 17β-雌二醇（E2）的情况。我们发现这些细胞中存在 HSD17B1 转录本和蛋白。此外，我们证明 Jurkat、Hut-78 和 Raji B 细胞能够从 E1 生成 E2。我们的结果表明 Jurkat 急性 T 细胞白血病、Hut-78 和 Raji B 淋巴瘤细胞能够在体外将弱雌激素 E1 转化为更有效的 E2。
