Poznań University of Medical Sciences, Department of Biochemistry and Molecular Biology, Poznań, Poland.
Biomed Pharmacother. 2012 Oct;66(7):530-4. doi: 10.1016/j.biopha.2012.02.006. Epub 2012 Mar 27.
It has recently been suggested that, in addition to genetic and environmental factors and tobacco exposure, estrogens also may be an independent risk factor in the development of lung cancer. Therefore, we evaluated the transcript and protein levels of 17-beta-hydroxysteroid-dehydrogenase type 1 (HSD17B1), and the conversion of estrone (E1) to 17-beta-estradiol (E2) in human non-small cell lung cancer (NSCLC) Calu-6, Calu-1 and A549 cells. In our work, we established the presence of HSD17B1 transcripts and proteins in all examined NSCLC cells. Moreover, we demonstrated that human NSCLC Calu-6, Calu-1 and A549 cells are able to convert weak estrogen E1 to highly potent E2 in vitro. Our results indicate that NSCLC cells are able to produce E2 from E1.
最近有人提出，除了遗传和环境因素以及烟草暴露外，雌激素也可能是肺癌发展的一个独立危险因素。因此，我们评估了 17-β-羟甾类脱氢酶 1 型（HSD17B1）的转录本和蛋白水平，以及人非小细胞肺癌（NSCLC）Calu-6、Calu-1 和 A549 细胞中雌酮（E1）向 17-β-雌二醇（E2）的转化。在我们的工作中，我们在所有检查的 NSCLC 细胞中都建立了 HSD17B1 转录本和蛋白的存在。此外，我们证明人 NSCLC Calu-6、Calu-1 和 A549 细胞能够在体外将弱雌激素 E1 转化为高活性的 E2。我们的结果表明，NSCLC 细胞能够从 E1 产生 E2。
