Institute for Biotechnology and Bioengineering (IBB), Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Can J Microbiol. 2013 Mar;59(3):183-8. doi: 10.1139/cjm-2012-0529. Epub 2012 Dec 19.
Filamentous fungi in drinking water can block water pipes, can cause organoleptic biodeterioration, and are a source of pathogens. There are increasing reports of the involvement of the organisms in biofilms. This present study describes a sampling device that can be inserted directly into pipes within water distribution systems, allowing biofilm formation in situ. Calcofluor White M2R staining and fluorescent in situ hybridization with morphological analyses using epifluorescent microscopy were used to analyse biofilms for filamentous fungi, permitting direct observation of the fungi. DAPI (4',6-diamidino-2-phenylindole) was applied to detect bacteria. Filamentous fungi were detected in biofilms after 6 months on coupons exposed to raw water, decanted water and at the entrance of the water distribution system. Algae, yeast, and bacteria were also observed. The role of filamentous fungi requires further investigations.
饮用水中的丝状真菌会阻塞水管，导致感官生物降解，并成为病原体的来源。越来越多的报告表明这些生物体参与了生物膜的形成。本研究描述了一种可直接插入供水系统管道内的采样装置，可在原位形成生物膜。使用 Calcofluor White M2R 染色和荧光原位杂交技术，并结合相差显微镜形态学分析，可用于分析丝状真菌生物膜，从而直接观察真菌。用 DAPI（4'，6-二脒基-2-苯基吲哚）检测细菌。在暴露于原水、沉淀池水和在供水系统入口处的样品上培养 6 个月后，在生物膜中检测到丝状真菌。还观察到藻类、酵母和细菌。丝状真菌的作用需要进一步研究。
