Alam T M, Drobny G
Department of Chemistry, University of Washington, Seattle 98195.
Biochemistry. 1990 Apr 10;29(14):3421-30. doi: 10.1021/bi00466a001.
Solid-state 2H NMR spectroscopy was used to investigate dynamics in the [methyl-2H]thymidine-labeled oligonucleotide [d(CGCGAATTCGCG)]2. Quadrupole echo line shapes, spin-lattice relaxation, and quadrupolar echo decay rates were investigated as a function of hydration W (moles of water/moles of nucleotide) between 0 and approximately 30. The amplitude of the base motion, modeled as a fast four-site libration, or diffusion in a cone, increased slightly with higher levels of hydration. A slower component of motion about the helix axis appears at W approximately 10 and increases in rate and amplitude, leading to the intermediate rate line shape observed at W approximately 21.
采用固态2H核磁共振光谱法研究了[甲基-2H]胸苷标记的寡核苷酸[d(CGCGAATTCGCG)]2中的动力学。研究了四极回波线形、自旋晶格弛豫和四极回波衰减率随水合度W（水的摩尔数/核苷酸的摩尔数）在0至约30之间的变化。碱基运动的幅度，模拟为快速四点摆动或圆锥体内的扩散，随着水合度的增加而略有增加。围绕螺旋轴的较慢运动分量在W约为10时出现，并在速率和幅度上增加，导致在W约为21时观察到中间速率的线形。
