C6位标记有¹³C的胸苷亚磷酰胺的合成：¹³C标记的DNA发夹中环区的弛豫研究。
Synthesis of a thymidine phosphoramidite labelled with 13C at C6: relaxation studies of the loop region in a 13C labelled DNA hairpin.
作者信息
Williamson J R, Boxer S G
机构信息
Department of Chemistry, Stanford University, CA 94305.
出版信息
Nucleic Acids Res. 1988 Feb 25;16(4):1529-40. doi: 10.1093/nar/16.4.1529.
Abstract
A thymidine phosphoramidite labelled at C6 with 13C has been synthesized, and incorporated into a synthetic oligonucleotide, d(CGCGTTGTTCGCG), which adopts a hairpin conformation. NMR relaxation measurements indicate that internal motion may be present in the loop region of the oligonucleotide. The relaxation behavior of a the C6 carbon in a model compound, N,N-1,3 dimethylthymine is examined in detail as a function of magnetic field strength to determine relative contributions of various mechanisms to the relaxation. The relaxation behaviour of the labelled carbons in the oligonucleotide is discussed in relation to these measurements.
摘要
已合成了在C6位用13C标记的胸苷亚磷酰胺，并将其掺入到一个合成的寡核苷酸d(CGCGTTGTTCGCG)中，该寡核苷酸呈发夹构象。核磁共振弛豫测量表明，该寡核苷酸的环区可能存在内部运动。详细研究了模型化合物N,N-1,3-二甲基胸腺嘧啶中C6碳的弛豫行为与磁场强度的关系，以确定各种机制对弛豫的相对贡献。结合这些测量结果讨论了寡核苷酸中标记碳的弛豫行为。
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