• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Synthesis of a thymidine phosphoramidite labelled with 13C at C6: relaxation studies of the loop region in a 13C labelled DNA hairpin.C6位标记有¹³C的胸苷亚磷酰胺的合成：¹³C标记的DNA发夹中环区的弛豫研究。
Nucleic Acids Res. 1988 Feb 25;16(4):1529-40. doi: 10.1093/nar/16.4.1529.
2
Preparation and heteronuclear 2D NMR spectroscopy of a DNA dodecamer containing a thymidine residue with a uniformly 13C-labeled deoxyribose ring.含一个具有均匀13C标记脱氧核糖环的胸苷残基的DNA十二聚体的制备及异核二维核磁共振波谱分析
J Biomol NMR. 1994 Jul;4(4):581-6. doi: 10.1007/BF00156622.
3
Multinuclear NMR studies of DNA hairpins. 1. Structure and dynamics of d(CGCGTTGTTCGCG).DNA发夹的多核核磁共振研究。1. d（CGCGTTGTTCGCG）的结构与动力学
Biochemistry. 1989 Apr 4;28(7):2819-31. doi: 10.1021/bi00433a012.
4
HCCCH experiment for through-bond correlation of thymine resonances in 13C-labeled DNA oligonucleotides.用于13C标记的DNA寡核苷酸中胸腺嘧啶共振的键连相关的HCCCH实验。
J Magn Reson. 1999 Apr;137(2):345-9. doi: 10.1006/jmre.1998.1687.
5
Dynamics in synthetic oligonucleotides. A solid-state deuterium NMR study.合成寡核苷酸的动力学。一项固态氘核磁共振研究。
Biochemistry. 1990 Apr 10;29(14):3421-30. doi: 10.1021/bi00466a001.
6
Oligodeoxynucleotides having a loop consisting of 3'-deoxy-4'-C-(2-hydroxyethyl)thymidines form stable hairpins.具有由3'-脱氧-4'-C-(2-羟乙基)胸苷组成的环的寡脱氧核苷酸形成稳定的发夹结构。
Biochemistry. 2004 Jul 13;43(27):8690-9. doi: 10.1021/bi0496283.
7
Base modification and cloning efficiency of oligodeoxyribonucleotides synthesized by the phosphoramidite method: methyl versus cyanoethyl phosphorus protection.亚磷酰胺法合成的寡脱氧核糖核苷酸的碱基修饰与克隆效率：甲基与氰乙基磷保护
Nucleic Acids Symp Ser. 1985(16):257-60.
8
Synthesis and characterization of oligodeoxynucleotides containing 4-O-methylthymine.含4-O-甲基胸腺嘧啶的寡脱氧核苷酸的合成与表征
Biochemistry. 1987 Feb 24;26(4):1086-93. doi: 10.1021/bi00378a015.
9
Synthesis and characterization of a [3-15N]-labeled cis-syn thymine dimer-containing DNA duplex.含[3-15N]标记的顺式-胸腺嘧啶二聚体的DNA双链体的合成与表征
J Org Chem. 2006 Feb 17;71(4):1640-6. doi: 10.1021/jo0524167.
10
13C-NMR relaxation in three DNA oligonucleotide duplexes: model-free analysis of internal and overall motion.三种DNA寡核苷酸双链体中的13C-NMR弛豫：内部和整体运动的无模型分析
Biochemistry. 1994 Mar 8;33(9):2441-50. doi: 10.1021/bi00175a012.

引用本文的文献

1
Isotope Labels Combined with Solution NMR Spectroscopy Make Visible the Invisible Conformations of Small-to-Large RNAs.同位素标记与溶液 NMR 光谱学联合使用，使小至大 RNA 的不可见构象可见。
Chem Rev. 2022 May 25;122(10):9357-9394. doi: 10.1021/acs.chemrev.1c00845. Epub 2022 Apr 20.
2
Synthesis and incorporation of 13C-labeled DNA building blocks to probe structural dynamics of DNA by NMR.合成并掺入13C标记的DNA构建模块以通过核磁共振探测DNA的结构动力学。
Nucleic Acids Res. 2017 Sep 6;45(15):9178-9192. doi: 10.1093/nar/gkx592.
3
Preparation of selective and segmentally labeled single-stranded DNA for NMR by self-primed PCR and asymmetrical endonuclease double digestion.通过自引发聚合酶链反应和不对称核酸内切酶双酶切制备用于核磁共振的选择性和片段标记的单链DNA
Nucleic Acids Res. 2009 Sep;37(17):e114. doi: 10.1093/nar/gkp540. Epub 2009 Jun 24.
4
Selectively 13C-enriched DNA: evidence from 13C1' relaxation rate measurements of an internal dynamics sequence effect in the lac operator.选择性富集13C的DNA：来自乳糖操纵子内部动力学序列效应的13C1'弛豫速率测量的证据。
J Biomol NMR. 1996 Oct;8(3):252-60. doi: 10.1007/BF00410324.
5
Preparation and heteronuclear 2D NMR spectroscopy of a DNA dodecamer containing a thymidine residue with a uniformly 13C-labeled deoxyribose ring.含一个具有均匀13C标记脱氧核糖环的胸苷残基的DNA十二聚体的制备及异核二维核磁共振波谱分析
J Biomol NMR. 1994 Jul;4(4):581-6. doi: 10.1007/BF00156622.
6
Structure of a small RNA hairpin.一种小RNA发夹结构。
Nucleic Acids Res. 1993 Feb 11;21(3):537-45. doi: 10.1093/nar/21.3.537.

本文引用的文献

1
Carbon 13 spin-lattice relaxation, linewidth, and nuclear Overhauser enhancement measurements of nucleosome length DNA.对核小体长度DNA进行碳13自旋晶格弛豫、线宽和核Overhauser增强测量。
J Biol Chem. 1981 Oct 10;256(19):9986-9.
2
Carbon-13 NMR relaxation studies of pre-melt structural dynamics in [4-13C-uracil] labeled E. coli transfer RNAIVal.[4-¹³C-尿嘧啶]标记的大肠杆菌转运RNAIVal中预熔结构动力学的碳-13核磁共振弛豫研究
Nucleic Acids Res. 1982 Jul 24;10(14):4449-64. doi: 10.1093/nar/10.14.4449.
3
Polymer support oligonucleotide synthesis XVIII: use of beta-cyanoethyl-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product.聚合物载体寡核苷酸合成 XVIII：脱氧核苷的β-氰基乙基-N，N-二烷基氨基-/N-吗啉代亚磷酰胺用于 DNA 片段合成，简化最终产物的脱保护和分离
Nucleic Acids Res. 1984 Jun 11;12(11):4539-57. doi: 10.1093/nar/12.11.4539.
4
An investigation by 1H NMR spectroscopy into the factors determining the beta:alpha ratio of the product in 2'-deoxynucleoside synthesis.通过¹H核磁共振光谱法对2'-脱氧核苷合成中决定产物β:α比例的因素进行的一项研究。
Nucleic Acids Res. 1984 Sep 11;12(17):6827-37. doi: 10.1093/nar/12.17.6827.
5
Conformation and dynamics of short DNA duplexes: (dC-dG)3 and (dC-dG)4.短DNA双链体的构象与动力学：(dC-dG)3和(dC-dG)4
J Biomol Struct Dyn. 1984 Jun;1(6):1373-86. doi: 10.1080/07391102.1984.10507526.
6
Internal dynamics of transfer ribonucleic acid determined by nuclear magnetic resonance of carbon-13-enriched ribose carbon 1.通过富含碳-13的核糖碳1的核磁共振确定的转移核糖核酸的内部动力学。
Biochemistry. 1983 Mar 15;22(6):1408-15. doi: 10.1021/bi00275a014.
7
Complete nuclear magnetic resonance signal assignments and initial structural studies of [13C]methyl-enriched yeast transfer ribonucleic acid.[13C]甲基富集酵母转移核糖核酸的完整核磁共振信号归属及初步结构研究
Biochemistry. 1983 Mar 15;22(6):1402-8. doi: 10.1021/bi00275a013.
8
Nuclear magnetic resonance signal assignments of purified [13C]methyl-enriched yeast phenylalanine transfer ribonucleic acid.纯化的富含[13C]甲基的酵母苯丙氨酸转移核糖核酸的核磁共振信号归属
Biochemistry. 1985 Mar 12;24(6):1434-40. doi: 10.1021/bi00327a023.
9
Natural abundance carbon-13 nuclear magnetic resonance studies of histone and DNA dynamics in nucleosome cores.核小体核心中组蛋白和DNA动力学的天然丰度碳-13核磁共振研究
J Biol Chem. 1986 May 5;261(13):5992-8.

C6位标记有¹³C的胸苷亚磷酰胺的合成：¹³C标记的DNA发夹中环区的弛豫研究。

Synthesis of a thymidine phosphoramidite labelled with 13C at C6: relaxation studies of the loop region in a 13C labelled DNA hairpin.

作者信息

Williamson J R, Boxer S G

机构信息

Department of Chemistry, Stanford University, CA 94305.

出版信息

Nucleic Acids Res. 1988 Feb 25;16(4):1529-40. doi: 10.1093/nar/16.4.1529.

DOI:10.1093/nar/16.4.1529
PMID:3347496
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC336332/
Abstract

A thymidine phosphoramidite labelled at C6 with 13C has been synthesized, and incorporated into a synthetic oligonucleotide, d(CGCGTTGTTCGCG), which adopts a hairpin conformation. NMR relaxation measurements indicate that internal motion may be present in the loop region of the oligonucleotide. The relaxation behavior of a the C6 carbon in a model compound, N,N-1,3 dimethylthymine is examined in detail as a function of magnetic field strength to determine relative contributions of various mechanisms to the relaxation. The relaxation behaviour of the labelled carbons in the oligonucleotide is discussed in relation to these measurements.

摘要

已合成了在C6位用13C标记的胸苷亚磷酰胺，并将其掺入到一个合成的寡核苷酸d(CGCGTTGTTCGCG)中，该寡核苷酸呈发夹构象。核磁共振弛豫测量表明，该寡核苷酸的环区可能存在内部运动。详细研究了模型化合物N,N-1,3-二甲基胸腺嘧啶中C6碳的弛豫行为与磁场强度的关系，以确定各种机制对弛豫的相对贡献。结合这些测量结果讨论了寡核苷酸中标记碳的弛豫行为。