Oh B H, Mooberry E S, Markley J L
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison 53706.
Biochemistry. 1990 Apr 24;29(16):4004-11. doi: 10.1021/bi00468a030.
Multinuclear two-dimensional NMR techniques were used to assign nearly all diamagnetic 13C and 15N resonances of the plant-type 2Fe.2S* ferredoxin from Anabaena sp. strain PCC 7120. Since a 13C spin system directed strategy had been used to identify the 1H spin systems [Oh, B.-H., Westler, W. M., & Markley, J. L. (1989) J. Am. Chem. Soc. 111, 3083-3085], the sequence-specific 1H assignments [Oh, B.-H., & Markley, J. L. (1990) Biochemistry (first paper of three in this issue)] also provided sequence-specific 13C assignments. Several resonances from 1H-13C groups were assigned independently of the 1H assignments by considering the distances between these nuclei and the paramagnetic 2Fe.2S* center. A 13C-15N correlation data set was used to assign additional carbonyl carbons and to analyze overlapping regions of the 13C-13C correlation spectrum. Sequence-specific assignments of backbone and side-chain nitrogens were based on 1H-15N and 13C-15N correlations obtained from various two-dimensional NMR experiments.
采用多核二维核磁共振技术对鱼腥藻7120菌株中植物型2Fe.2S铁氧化还原蛋白几乎所有抗磁性的13C和15N共振峰进行了归属。由于之前已采用13C自旋系统导向策略来识别1H自旋系统[Oh, B.-H., Westler, W. M., & Markley, J. L. (1989) J. Am. Chem. Soc. 111, 3083 - 3085]，所以序列特异性的1H归属[Oh, B.-H., & Markley, J. L. (1990) Biochemistry（本期三篇论文中的第一篇）]也提供了序列特异性的13C归属。通过考虑这些原子核与顺磁性2Fe.2S中心之间的距离，独立于1H归属对来自1H-13C基团的几个共振峰进行了归属。利用一个13C-15N相关数据集来归属额外的羰基碳，并分析13C-13C相关谱的重叠区域。主链和侧链氮原子的序列特异性归属基于从各种二维核磁共振实验获得的1H-15N和13C-15N相关。
