Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA.
J Neurosci Methods. 2013 Sep 15;218(2):206-13. doi: 10.1016/j.jneumeth.2013.03.004. Epub 2013 Mar 29.
A major challenge in the histological sectioning of brain tissue is achieving accurate alignment in the standard coronal, horizontal, or sagittal planes. Correct alignment is desirable for ease of subsequent analysis and is a prerequisite for computational registration and algorithm-based quantification of experimental data. We have developed a simple and low-cost technique for whole-brain cryosectioning of rodent brains that reliably results in a precise alignment of stereotactic coordinates. The system utilises a 3-D printed model of a mouse brain to create a tailored cavity that is used to align and support the brain during freezing. The alignment of the frozen block is achieved in relation to the fixed edge of the mold. The system also allows for two brains to be frozen and sectioned simultaneously. System components, procedural steps, and examples of the end results are presented.
脑组织的组织学切片的主要挑战是在标准的冠状、水平或矢状平面上实现精确的对准。准确的对准便于后续的分析，也是计算配准和基于算法的实验数据定量的前提。我们开发了一种简单且低成本的方法，用于对啮齿动物大脑进行全脑冷冻切片，可可靠地实现精确的立体定向坐标对准。该系统利用小鼠大脑的 3D 打印模型创建一个定制的腔，用于在冷冻过程中对齐和支撑大脑。冷冻块的对准与模具的固定边缘有关。该系统还允许同时冷冻和切片两个大脑。本文介绍了系统组件、程序步骤和最终结果的示例。
