Department of Biochemistry and Nutrition, CSIR-Central Food Technological Research Institute, Mysore, 570 020, Karnataka, India.
Eur J Nutr. 2014 Feb;53(1):117-26. doi: 10.1007/s00394-013-0507-9. Epub 2013 Mar 30.
To investigate the influence of olive (OO), groundnut (GNO), soybean (SBO), sunflower (SFO), rice bran (RBO), corn (CO), palm (PO) oil or mixed micelle (control) on absorption kinetics and bioavailability of lutein in lutein-deficient mice. Additional aim was to correlate the activity of intestinal triacylglycerol lipase with intestinal and plasma lutein levels.
After induction of lutein deficiency, mice (n = 165) were divided into eight groups (OO, SFO, GNO, RBO, PO, CO, SBO and control; n = 20/group) and the remaining (n = 5) were used as baseline (0 h). Groups were further divided into four subgroups (n = 5/subgroup) and were intubated with lutein (200 μM) dispersed in different vegetable oils. Plasma and tissue (intestine, liver and eyes), lutein, triglycerides, intestinal triacylglycerol lipases and fatty acid profile of plasma and tissues were measured at different time intervals.
The percentage area under the curve value for plasma lutein in OO and GNO was higher by 41.8 and 5.1 %, while it was lower in other groups (18.2-53.3 %), when compared to control. Similarly, the percentage area under the curve for eye lutein in OO and GNO groups was higher by 35.2 and 4.8 %, whereas in other groups it was lower (5.4-69 %) than in control. Results show that olive oil facilitates the lutein absorption more compared to other vegetable oils, which may be due to the difference in fatty acid composition and higher activity of intestinal triacylglycerol lipase.
Dietary olive oil rich in oleic acid improves the bioavailability and accumulation of lutein in lutein-deficient mice by modifying the intestinal triacylglycerol lipase activity.
研究橄榄油（OO）、花生油（GNO）、大豆油（SBO）、葵花籽油（SFO）、米糠油（RBO）、玉米油（CO）、棕榈油（PO）或混合胶束（对照）对叶黄素缺乏症小鼠叶黄素吸收动力学和生物利用度的影响。另一个目的是将肠道三酰基甘油脂肪酶的活性与肠道和血浆叶黄素水平相关联。
在诱导叶黄素缺乏后，将小鼠（n = 165）分为 8 组（OO、SFO、GNO、RBO、PO、CO、SBO 和对照；n = 20/组），其余（n = 5）作为基线（0 h）。将这些组进一步分为 4 个亚组（n = 5/亚组），并通过不同植物油分散的叶黄素（200 μM）进行插管。在不同时间间隔测量血浆和组织（肠道、肝脏和眼睛）、叶黄素、甘油三酯、肠道三酰基甘油脂肪酶以及血浆和组织的脂肪酸谱。
与对照组相比，OO 和 GNO 组的血浆叶黄素曲线下面积百分比值增加了 41.8%和 5.1%，而其他组则降低了 18.2%-53.3%。同样，OO 和 GNO 组眼睛中叶黄素的曲线下面积百分比值增加了 35.2%和 4.8%，而其他组则降低了 5.4%-69%。结果表明，橄榄油比其他植物油更能促进叶黄素的吸收，这可能是由于脂肪酸组成的差异和肠道三酰基甘油脂肪酶活性更高所致。
富含油酸的膳食橄榄油通过改变肠道三酰基甘油脂肪酶的活性，提高了叶黄素缺乏症小鼠中叶黄素的生物利用度和积累。
