Max Planck Institute for Polymer Research, Mainz, Germany.
Anal Chem. 2013 May 7;85(9):4307-10. doi: 10.1021/ac4003479. Epub 2013 Apr 18.
Near-edge X-ray absorption fine structure (NEXAFS) spectroscopy, as a technique, offers detailed information about the bonding environment of molecules at a surface. However, because it is a synchrotron based method, beam-time is limited and users must typically prioritize and narrowly define the scopes of experiments. In this study, we demonstrate a novel method that opens up the possibility of the use of large area NEXAFS imaging to pursue combinatorial studies. To explore the capabilities of the NIST full field NEXAFS microscope available at the National Synchrotron Light Source as a high throughput imaging instrument, we collected NEXAFS images from a sample array consisting of 144 different elements with a periodic sequence of different surface modifications. NEXAFS images collected from this model system illustrate how hyperspectral NEXAFS data can be used for parallel analysis of large numbers of samples either directly from the overall image or by extracting spectra from regions of interest.
近边 X 射线吸收精细结构（NEXAFS）光谱学是一种技术，可提供有关表面分子键合环境的详细信息。但是，由于它是基于同步加速器的方法，束时间有限，用户通常必须优先考虑并狭义地定义实验范围。在这项研究中，我们展示了一种新方法，为使用大面积 NEXAFS 成像进行组合研究开辟了可能性。为了探索美国国家标准与技术研究院（NIST）全场 NEXAFS 显微镜在作为高通量成像仪器方面的功能，我们从一个由 144 种不同元素组成的样品阵列中收集了 NEXAFS 图像，该阵列具有不同表面修饰的周期性序列。从该模型系统收集的 NEXAFS 图像说明了如何直接从整体图像或通过从感兴趣区域提取光谱，使用高光谱 NEXAFS 数据对大量样本进行平行分析。
