Huaiyin Institute of Technology, Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaian 223001, China; Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, Huaian 223300, China; Huaian High-Tech Research Institute of Nanjing University, Huaian 223001, China.
Carbohydr Polym. 2014 Jan;99:1-10. doi: 10.1016/j.carbpol.2013.07.088. Epub 2013 Aug 17.
Ultrasonic-microwave synergistic extraction (UMSE), purification, and characterization of Tricholoma mongolicum Imai polysaccharides (TMIPs) from fruit bodies were investigated in this study. Response surface methodology (RSM) was employed to optimize the UMSE conditions. The results indicated that the optimal extraction conditions were as follows: extraction time of 24.65 min, microwave power of 109.98 W, and water to raw material ratio of 21.62 ml/g. Under optimized conditions, the yield and purity of TMIPs were 35.41 ± 0.62% and 73.92 ± 0.83%, respectively. Crude TMIPs were purified by DEAE-Cellulose 52 chromatography and Sephadex G-100 chromatography to afford four fractions, namely, TMIP-1, TMIP-2, TMIP-3, and TMIP-4. Preliminary TMIP characterization was conducted by Fourier transform infrared spectroscopy. TMIP antioxidant activities were investigated by measuring its scavenging ability on 2,2-diphenyl-1-picrylhydrazyl, hydroxyl radicals, ferric reducing activity power, and reducing power assay. The results indicated that TMIPs have good antioxidant activity.
本研究采用超声-微波协同提取（UMSE）、纯化和鉴定从子实体中提取的蒙古口蘑多糖（TMIPs）。响应面法（RSM）被用于优化 UMSE 条件。结果表明，最佳提取条件为：提取时间 24.65 分钟，微波功率 109.98W，水与原料比为 21.62ml/g。在优化条件下，TMIPs 的得率和纯度分别为 35.41±0.62%和 73.92±0.83%。粗 TMIPs 通过 DEAE-纤维素 52 层析和 Sephadex G-100 层析纯化，得到四个组分，分别为 TMIP-1、TMIP-2、TMIP-3 和 TMIP-4。采用傅里叶变换红外光谱对 TMIP 进行了初步鉴定。通过测定其对 2,2-二苯基-1-苦基肼、羟基自由基、铁还原能力和还原能力的清除能力，研究了 TMIP 的抗氧化活性。结果表明，TMIPs 具有良好的抗氧化活性。
