两种生物膜形成弧菌属的胞外聚合物质：特性与应用。
Extracellular polymeric substances from two biofilm forming Vibrio species: characterization and applications.
机构信息
Discipline of Marine Biotechnology and Ecology, CSIR-Central Salt and Marine Chemicals Research Institute, G.B. Marg, Bhavnagar 364 002, Gujarat, India.
出版信息
Carbohydr Polym. 2013 May 15;94(2):882-8. doi: 10.1016/j.carbpol.2013.02.010. Epub 2013 Feb 18.
The early biofilm colonizing Vibrio campbellii and Vibrio fortis produced 400 and 134 mg L(-1) extracellular polymeric substances (EPS), respectively of distinct composition in planktonic cultures. The EPS of V. campbellii consisted of five monosaccharides: arabinose, galactose, glucose, mannose and rhamnose, however, that of V. fortis has only three monosaccharides: arabinose, galactose and mannose. The AFM topography analysis showed different morphology and physical characteristics of EPSs. FTIR and NMR spectral analyses revealed characteristic functional groups of the polysaccharides. EPSs were amorphous in nature and contained characteristic diffraction peaks. EPSs showed high emulsifying activity and pseudoplastic rheology. This is the first report of detailed characterization of EPS produced by V. campbellii and V. fortis, so far. Detailed analytical (FT-IR, (1)H NMR, PXRD, etc.) and bio-physicochemical characteristics (thermostability, emulsifying and rheological properties) of EPSs showed potential for industrial applications.
早期生物膜定殖的坎贝尔氏弧菌和福氏弧菌分别在浮游培养物中产生了 400 和 134 mg/L 的胞外聚合物（EPS），其组成明显不同。坎贝尔氏弧菌的 EPS 由五种单糖组成：阿拉伯糖、半乳糖、葡萄糖、甘露糖和鼠李糖，而福氏弧菌的 EPS 仅由三种单糖组成：阿拉伯糖、半乳糖和甘露糖。AFM 形貌分析显示 EPS 的形态和物理特性不同。FTIR 和 NMR 光谱分析揭示了多糖的特征功能基团。EPS 本质上是无定形的，含有特征衍射峰。EPS 具有高乳化活性和伪塑性流变学特性。这是迄今为止首次对坎贝尔氏弧菌和福氏弧菌产生的 EPS 进行详细表征的报道。EPS 的详细分析（FT-IR、（1）H NMR、PXRD 等）和生物物理化学特性（热稳定性、乳化和流变特性）显示出了在工业应用方面的潜力。
Zotero 插件