Long Dongyan, Hashmi Muhammad Zaffar, Su Xiaomei, Pongpiachan Siwatt
1College of Environmental and Natural Resource Sciences, Zhejiang University, Yuhangtang Road 388, Hangzhou, 310058 Zhejiang People's Republic of China.
Chaotianmen Sub-district Office, Yuzhong District, Chongqing, 400011 People's Republic of China.
3 Biotech. 2019 Mar;9(3):111. doi: 10.1007/s13205-019-1641-8. Epub 2019 Feb 28.
A better understanding of the Cr(VI) reduction position and mechanisms by a Cr(VI)-reducing strain is important for the bioremediation of Cr pollution in the environment. In the present study, we were interested in figuring out the role of extracellular polymeric substances (EPS) as the main area for Cr(VI) reduction in the newly reported strain of LY10. We investigated the subcellular distribution and reduction capability of each cellular component as the main area of Cr(VI) reduction by scanning electron microscopy and soft X-ray spectromicroscopy. The results suggested that most of Cr was presented in the supernatants as Cr(III) after reduction. In the cells, Cr was mostly distributed in the EPS and cell wall, while the EPS had the maximum Cr(VI) reduction rate (81.5%) as compared with the cell wall (30.1%). Soft X-ray spectromicroscopy analysis indicated that Cr accumulated more in the EPS. Therefore, the results suggested that the EPS were the main area for Cr(VI) reduction in the bacteria of LY10.
深入了解六价铬还原菌株对六价铬的还原位置和机制,对于环境中铬污染的生物修复至关重要。在本研究中,我们旨在弄清新报道的LY10菌株中细胞外聚合物(EPS)作为六价铬还原主要区域所起的作用。我们通过扫描电子显微镜和软X射线光谱显微镜研究了作为六价铬还原主要区域的每个细胞成分的亚细胞分布和还原能力。结果表明,还原后大部分铬以三价铬形式存在于上清液中。在细胞中,铬主要分布在EPS和细胞壁中,而与细胞壁(30.1%)相比,EPS具有最高的六价铬还原率(81.5%)。软X射线光谱显微镜分析表明,铬在EPS中积累更多。因此,结果表明EPS是LY10菌株中六价铬还原的主要区域。