Chang Chia-Hao, Shao Kwang-Tsao, Lin Yeong-Shin, Liao Yun-Chih
Biodiversity Research Center, Academia Sinica , Taiwan , ROC.
Mitochondrial DNA. 2013 Dec;24(6):665-7. doi: 10.3109/19401736.2013.773321. Epub 2013 Apr 2.
The complete mitochondrial genome of the three-spot seahorse was sequenced using a polymerase chain reaction-based method. The total length of mitochondrial DNA is 16,535 bp and includes 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and a control region. The mitochondrial gene order of the three-spot seahorse also conforms to the distinctive vertebrate mitochondrial gene order. The base composition of the genome is A (32.7%), T (29.3%), C (23.4%), and G (14.6%) with an A + T-rich hallmark as that of other vertebrate mitochondrial genomes.
采用基于聚合酶链反应的方法对三斑海马的完整线粒体基因组进行了测序。线粒体DNA的总长度为16535碱基对,包括13个蛋白质编码基因、2个核糖体RNA基因、22个转运RNA基因和一个控制区。三斑海马的线粒体基因排列也符合独特的脊椎动物线粒体基因排列。基因组的碱基组成为A(32.7%)、T(29.3%)、C(23.4%)和G(14.6%) -- 具有其他脊椎动物线粒体基因组中富含A+T的特征。