Linderman J J, Harris L J, Slakey L L, Gross D J
Department of Chemical Engineering, University of Michigan, Ann Arbor.
Cell Calcium. 1990 Feb-Mar;11(2-3):131-44. doi: 10.1016/0143-4160(90)90066-4.
Transient changes in the concentration of intracellular free calcium are associated with the transduction of primary signals and the subsequent employment of Ca2+ as a second messenger in a multitude of cell types. These transients, typically monitored with the calcium-sensitive fluorescent dye Fura-2, are known to occur with a time course in the order of seconds. In order to accurately monitor such rapid changes in intracellular free calcium concentration in both single cells and simultaneously in several cells in a single field, we have developed a digital fluorescence imaging system based on a charge-coupled device (CCD) camera. We report here on the detailed kinetics of calcium increases in cultured arterial swine smooth muscle cells in response to the agonist ATP.
