Chulabhorn Research Institute, Bangkok, Thailand.
Int J Hyg Environ Health. 2013 Aug;216(5):566-73. doi: 10.1016/j.ijheh.2013.03.003. Epub 2013 Mar 15.
A recent focus has been targeted toward the development of functional biomarkers that can be used to predict disease more reliably. One such biomarker is the challenge assay for DNA repair deficiency. Briefly, the assay involves challenging lymphocytes in culture to a DNA damaging agent in vitro and determining the repair outcome in chromosome aberrations and/or DNA strand breaks. The aim is to show that individuals who have chronic exposure to toxic substances will develop exposure-induced DNA repair deficiencies. Many studies around the world have shown that the assay detects DNA repair deficiency in environmentally/occupationally exposed populations and with significant exposure dose-response relationship. The prediction of health risk was also validated. In addition, exposure-induced repair deficiency which was apparently passed through the germ cells had caused genetic consequences in a 3-generation population. The assay is simple to conduct and is more sensitive than some traditional biomarker assays. Together with the functional significance of the assay, the challenge assay can be used with confidence in population studies for health risk assessment.
最近的研究重点集中在开发功能生物标志物上，这些标志物可以更可靠地用于预测疾病。其中一种生物标志物是 DNA 修复缺陷的挑战检测。简要地说，该检测涉及在体外用 DNA 损伤剂刺激培养中的淋巴细胞，并确定染色体畸变和/或 DNA 链断裂的修复结果。其目的是表明长期接触有毒物质的个体将发展出暴露诱导的 DNA 修复缺陷。世界各地的许多研究表明，该检测可以在暴露于环境/职业有毒物质的人群中检测到 DNA 修复缺陷，并且与显著的暴露剂量反应关系有关。健康风险的预测也得到了验证。此外，暴露诱导的修复缺陷显然通过生殖细胞传递，导致了三代人群的遗传后果。该检测方法简单易行，比一些传统的生物标志物检测方法更敏感。结合该检测的功能意义，在人群健康风险评估的研究中可以放心使用该挑战检测。
