Centre of Molecular and Environmental Biology, Department of Biology, University of Minho, Braga 4710-057, Portugal.
J Microbiol Methods. 2013 Jun;93(3):206-8. doi: 10.1016/j.mimet.2013.03.016. Epub 2013 Mar 29.
We herein present a high-throughput and cheap method for yeast DNA isolation in a 96 well microplate. About 1500 yeast isolates can be processed within one working day and final DNA concentrations are suitable for direct application in PCR-based molecular typing methods.
我们在此介绍了一种高通量且廉价的方法,用于在 96 孔微量离心管中分离酵母 DNA。大约 1500 个酵母分离株可以在一天内处理完成,最终的 DNA 浓度适用于直接应用于基于 PCR 的分子分型方法。