Departamento de Ciencias Biomédicas (Area de Microbiología), Facultad de Ciencias (Antiguo Rectorado), Universidad de Extremadura, 06071 Badajoz, Spain.
Food Microbiol. 2010 Apr;27(2):205-9. doi: 10.1016/j.fm.2009.10.004. Epub 2009 Oct 9.
Quick and accurate methods are required for the identification of industrial, environmental, and clinical yeast strains. We propose a rapid method for the simultaneous extraction of yeast mtDNA, nuclear DNA, and virus dsRNA. It is simpler, cheaper, and faster than the previously reported methods. It allows one to choose among a broad range of molecular analysis approaches for yeast typing, avoiding the need to use of several different methods for the separate extraction of each nucleic acid type. The application of this method followed by the combined analysis of mtDNA and dsRNA (ScV-M and W) is a highly attractive option for fast and efficient wine yeast typing.
需要快速准确的方法来鉴定工业、环境和临床酵母菌株。我们提出了一种同时提取酵母 mtDNA、核 DNA 和病毒 dsRNA 的快速方法。与以前报道的方法相比，该方法更简单、更便宜、更快。它允许人们在广泛的分子分析方法中选择用于酵母分型的方法，避免了使用几种不同的方法分别提取每种核酸类型的需要。这种方法的应用，再加上 mtDNA 和 dsRNA（ScV-M 和 W）的联合分析，是快速高效葡萄酒酵母分型的极具吸引力的选择。
