Zhang Z Y, Pang X M, Han J W, Wang Y, Li Y Y
National Engineering Laboratory for Tree Breeding of the National Development and Reform Commission, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.
Genet Mol Res. 2013 Mar 11;12(3):3965-74. doi: 10.4238/2013.March.11.9.
The endangered perennial plant Annamocarya sinensis (Dode) Leroy is a tertiary relict tree restricted to southeastern China and northern Vietnam. To explore endangerment mechanisms, develop protection strategies, and guide reintroduction efforts for this species, we investigated genetic diversity and population structure by surveying 70 individuals from three distinct populations using 12 polymorphic microsatellite markers. We found high genetic diversity for A. sinensis as indicated by high allelic diversity (allelic number = 4.667 ± 0.436, effective number of alleles = 2.913 ± 0.249), excess heterozygosity (observed heterozygosity = 0.586 ± 0.039, expected heterozygosity = 0.582 ± 0.029), and low fixation index (-0.028 ± 0.057). Our research revealed low genetic differentiation (FST = 0.066 ± 0.011) and no correlation between genetic distance and geographic distance. Analysis of molecular variance attributed 87% of the variance to differences within the population, whereas 13% was distributed among populations. The protection strategy should aim to protect as many populations as possible. Promoting sexual reproduction among various genotypes and establishing an outcrossing program are advisable for A. sinensis.
濒危多年生植物蒜头果（Annamocarya sinensis (Dode) Leroy）是一种第三纪孑遗树种，仅分布于中国东南部和越南北部。为探究该物种的濒危机制、制定保护策略并指导其迁地保护工作，我们利用12个多态性微卫星标记对来自三个不同种群的70个个体进行了调查，以研究其遗传多样性和种群结构。我们发现，蒜头果具有较高的遗传多样性，表现为较高的等位基因多样性（等位基因数 = 4.667 ± 0.436，有效等位基因数 = 2.913 ± 0.249）、过量杂合性（观察杂合度 = 0.586 ± 0.039，期望杂合度 = 0.582 ± 0.029）以及较低的固定指数（-0.028 ± 0.057）。我们的研究还揭示了较低的遗传分化（FST = 0.066 ± 0.011），且遗传距离与地理距离之间无相关性。分子方差分析表明，87%的变异源自种群内部差异，而13%的变异分布于种群之间。保护策略应旨在尽可能多地保护种群。对于蒜头果而言，促进不同基因型间的有性繁殖并建立异交计划是可取的。
Zotero 插件