Luo Jie, Chen Junhao, Guo Wenlei, Yang Zhengfu, Lim Kean-Jin, Wang Zhengjia
State Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A&F University, Lin'an, Hangzhou 311300, China.
Department of Biology, Saint Louis University, St. Louis, MO 63104, USA.
Plants (Basel). 2021 Dec 24;11(1):52. doi: 10.3390/plants11010052.
Due to its peculiar morphological characteristics, there is dispute as to whether the genus of , a species of Juglandaceae, is or . Most morphologists believe it should be distinguished from the genus while genomicists suggest that belongs to the genus. To explore the taxonomic status of using chloroplast genes, we collected chloroplast genomes of 16 plant species and assembled chloroplast genomes of 10 unpublished species. We analyzed all 26 species' chloroplast genomes through two analytical approaches (concatenation and coalescence), using the entire and unique chloroplast coding sequence (CDS) and entire and protein sequences. Our results indicate that the analysis of the CDS and protein sequences or unique CDS and unique protein sequence of chloroplast genomes shows that indeed belongs to the genus. In addition, our analysis shows that, compared to single chloroplast genes, the phylogeny trees constructed using numerous genes showed higher consistency. Moreover, the phylogenetic analysis calculated with the coalescence method and unique gene sequences was more robust than that done with the concatenation method, particularly for analyzing phylogenetically controversial species. Through the analysis, our results concluded that should be called .
由于其独特的形态特征，胡桃科的一个物种，关于它属于 属还是 属存在争议。大多数形态学家认为它应与 属区分开来，而基因组学家则认为 属于 属。为了利用叶绿体基因探索 的分类地位，我们收集了16种植物的叶绿体基因组，并组装了10个未发表的 物种的叶绿体基因组。我们通过两种分析方法（串联和合并）分析了所有26个物种的叶绿体基因组，使用了完整和独特的叶绿体编码序列（CDS）以及完整和蛋白质序列。我们的结果表明，对叶绿体基因组的CDS和蛋白质序列或独特CDS和独特蛋白质序列的分析表明， 确实属于 属。此外，我们的分析表明，与单个叶绿体基因相比，使用多个基因构建的系统发育树显示出更高的一致性。而且，用合并方法和独特基因序列进行的系统发育分析比用串联方法更稳健，特别是对于分析系统发育有争议的物种。通过分析，我们的结果得出结论， 应称为 。
