Ostrovsky Aaron, Cachero Sebastian, Jefferis Gregory
Cold Spring Harb Protoc. 2013 Apr 1;2013(4):342-6. doi: 10.1101/pdb.prot071720.
Clonal analysis with the MARCM (mosaic analysis with a repressible cell marker) system can be used for studying cell lineage, development, and anatomy in the Drosophila olfactory system and other parts of the fly brain. This protocol describes the dissection, staining, and imaging of brains from Drosophila with mosaic labeling. Staining for the presynaptic marker Bruchpilot (nc82) is performed in the example given here. The well-stained whole brain images that are obtained can be used to examine neuronal morphology. They are of sufficient quality to be used for image registration, which allows one to compare confocal images of labeled neurons in different brains.
利用MARCM（带有可抑制细胞标记的镶嵌分析）系统进行的克隆分析可用于研究果蝇嗅觉系统及果蝇大脑其他部位的细胞谱系、发育和解剖结构。本方案描述了对具有镶嵌标记的果蝇大脑进行解剖、染色和成像的方法。此处给出的示例中对突触前标记Bruchpilot（nc82）进行了染色。获得的染色良好的全脑图像可用于检查神经元形态。它们的质量足以用于图像配准，从而能够比较不同大脑中标记神经元的共聚焦图像。
