Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University, Xi'an 710069, China.
Brain Res. 2013 May 28;1512:89-96. doi: 10.1016/j.brainres.2013.03.041. Epub 2013 Mar 30.
Subarachnoid hemorrhage (SAH) is a frequent occurrence in cerebrovascular accidents, and inflammation occurs in the subarachnoid space after SAH. Arachnoid cells have the capability to present antigens and active T-lymphocytes after stimulation by cerebrospinal fluid (CSF). However, the effect of CSF on T-lymphocytes and arachnoid cell adhesion was not clearly understood. In this study, we used ELISA to detected tumor necrosis factor-α (TNF-α) content in CSF of SAH patients. CSF or recombinant TNF-α were applied on arachnoid cells and T-lymphoctes, and RT-PCR and western blotting were performed to determine the expression of intercellular adhesion molecule-1 (ICAM-1) in arachnoid cells and Lymphocyte Function-Associated Antigen-1 (LFA-1) in T-lymphocytes, respectively. Meanwhile, the Matrix Metal Proteinase-9 (MMP-9) expression in these cells was also determined. We found that the content of TNF-α in the CSF was significantly increased in the CSF of SAH patients (from 22 ± 8 pg/mL of healthy people to 436-450 pg/mL of SAH patients). Treatement with CSF could increase the expression of ICAM-1 in arachnoid cells and that of LFA-1 in T-lymphocytes, mainly through the increased levels of TNF-α. We also found that the co-culture of arachnoid cells and T-lymphocytes increased the expression of MMP-9 in both cells through the interaction of ICAM-1 of and LFA-1. All of these results suggested that arachnoid cells are involved in the T-lymphocytes invasion in the subarachnoid space after SAH.
蛛网膜下腔出血（SAH）是脑血管意外的常见事件，SAH 后蛛网膜下腔发生炎症。蛛网膜细胞在受到脑脊液（CSF）刺激后具有呈递抗原和激活 T 淋巴细胞的能力。然而，CSF 对 T 淋巴细胞和蛛网膜细胞黏附的影响尚不清楚。在本研究中，我们使用 ELISA 检测 SAH 患者 CSF 中的肿瘤坏死因子-α（TNF-α）含量。将 CSF 或重组 TNF-α应用于蛛网膜细胞和 T 淋巴细胞，通过 RT-PCR 和 Western blot 分别检测蛛网膜细胞中细胞间黏附分子-1（ICAM-1）和 T 淋巴细胞中淋巴细胞功能相关抗原-1（LFA-1）的表达，同时检测这些细胞中基质金属蛋白酶-9（MMP-9）的表达。结果发现，SAH 患者 CSF 中 TNF-α含量明显升高（从健康人 22±8pg/ml 升高至 436-450pg/ml）。CSF 处理可增加蛛网膜细胞中 ICAM-1的表达和 T 淋巴细胞中 LFA-1的表达，主要通过增加 TNF-α水平。我们还发现，蛛网膜细胞和 T 淋巴细胞的共培养通过 ICAM-1 和 LFA-1 的相互作用增加了两种细胞中 MMP-9 的表达。这些结果表明，蛛网膜细胞参与了 SAH 后 T 淋巴细胞在蛛网膜下腔的浸润。
