Prachi P, Donati C, Masciopinto F, Rappuoli R, Bagnoli F
Novartis Vaccines, Research Center, Siena, Italy.
Public Health Genomics. 2013;16(1-2):62-8. doi: 10.1159/000345611. Epub 2013 Mar 18.
Vaccine research has experienced a quantum leap after the beginning of the genomics era. High-throughput sequencing techniques, unlimited computing resources, as well as new bioinformatic algorithms are now changing the way we perform genomic studies. Whole genome sequencing will soon become the gold standard for phylogenetic and epidemiology studies and is already shedding new light on the dynamics of bacterial evolution. We believe that deep sequencing projects, together with structural studies on vaccine candidates, will allow targeting constant epitopes and avoid vaccine failure due to antigenic variability. Systems biology, which is expected to revolutionize vaccine research and clinical studies, greatly relies on high-throughput technologies such as RNA-seq. Furthermore, genomics is a key element to develop safer vaccines, and the accuracy of deep sequencing will allow monitoring vaccine coverage after their introduction on the market.
基因组学时代开启后，疫苗研究实现了巨大飞跃。高通量测序技术、无限的计算资源以及新的生物信息学算法正在改变我们进行基因组研究的方式。全基因组测序很快将成为系统发育和流行病学研究的金标准，并且已经为细菌进化动态带来了新的认识。我们相信，深度测序项目，连同对候选疫苗的结构研究，将能够靶向恒定表位并避免因抗原变异性导致的疫苗失效。系统生物学有望革新疫苗研究和临床研究，它极大地依赖于诸如RNA测序等高通量技术。此外，基因组学是开发更安全疫苗的关键要素，深度测序的准确性将有助于监测疫苗上市后的接种覆盖率。
