从着丝粒上清除 PLK1。

Cullin' PLK1 from kinetochores.

机构信息

Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA.

出版信息

Nat Cell Biol. 2013 Apr;15(4):347-8. doi: 10.1038/ncb2722.

Abstract

To ensure proper attachment of all chromosomes to the spindle, PLK1 has to associate with kinetochores during prometaphase and must be released from these sites before sister chromatid separation can begin. The monoubiquitylation of PLK1 by the ubiquitin ligase CUL3-KLHL22 is now identified as a critical step in promoting the release of PLK1 from kinetochores, pushing non-proteolytic ubiquitylation into the limelight of cell division research.

摘要

为确保所有染色体正确连接到纺锤体上，PLK1 在前期必须与着丝粒结合，并且在姐妹染色单体分离开始之前必须从这些位点释放。现在已经确定，泛素连接酶 CUL3-KLHL22 对 PLK1 的单泛素化是促进 PLK1 从着丝粒释放的关键步骤，将非蛋白水解泛素化推到了细胞分裂研究的前沿。