• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

玻璃化对中期 II 期小鼠卵母细胞中启动子 CpG 岛甲基化模式以及 DNA 甲基转移酶 1o、组蛋白乙酰转移酶 1 和去乙酰化酶 1 表达水平的影响。

Effect of vitrification on promoter CpG island methylation patterns and expression levels of DNA methyltransferase 1o, histone acetyltransferase 1, and deacetylase 1 in metaphase II mouse oocytes.

机构信息

Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.

出版信息

Fertil Steril. 2013 Jul;100(1):256-61. doi: 10.1016/j.fertnstert.2013.03.009. Epub 2013 Mar 30.

DOI:10.1016/j.fertnstert.2013.03.009
PMID:23548937
Abstract

OBJECTIVE

To investigate the effect of vitrification on Dnmt1o, Hat1, and Hdac1 promoter CpG island methylation patterns and messenger RNA (mRNA) expression levels in mouse metaphase II (MII) oocytes.

DESIGN

In vitro study.

SETTING

Academic institution.

ANIMAL(S): Kunming white mice.

INTERVENTION(S): After vitrification, surviving mouse MII oocytes subjected to methylation and expression analysis with fresh oocytes used as a control.

MAIN OUTCOME MEASURE(S): Expression levels of mRNA as measured by real-time reverse-transcriptase polymerase chain reaction of methylation patterns of the CpG islands in the Dnmt1o, Hat1, and Hdac1 promoters analyzed by bisulfite mutagenesis and sequencing.

RESULT(S): The methylation patterns of the promoter CpG islands in Dnmt1o, Hat1, and Hdac1 were not statistically significantly when comparing vitrified oocytes and fresh oocytes. The expression levels of Hat1 and Hdac1 mRNA were not statistically significantly different in comparing in vitrified oocytes and fresh oocytes. The expression of Dnmt1o mRNA was statistically significantly lower in vitrified oocytes compared with fresh oocytes.

CONCLUSION(S): Vitrification did not statistically significantly alter the methylation patterns of the promoter CpG islands in Dnmt1o, Hat1, or Hdac1, but did statistically significantly decrease the expression of Dnmt1o mRNA in mouse MII oocytes.

摘要

目的

研究玻璃化对 Dnmt1o、Hat1 和 Hdac1 启动子 CpG 岛甲基化模式和信使 RNA（mRNA）表达水平的影响在小鼠中期 II（MII）卵母细胞中的作用。

设计

体外研究。

地点

学术机构。

动物

昆明小白鼠。

干预

玻璃化后，对存活的小鼠 MII 卵母细胞进行甲基化和表达分析，以新鲜卵母细胞作为对照。

主要观察指标

用实时逆转录聚合酶链反应测定 Dnmt1o、Hat1 和 Hdac1 启动子 CpG 岛甲基化模式的 mRNA 表达水平，并用亚硫酸氢盐诱变和测序进行分析。

结果

Dnmt1o、Hat1 和 Hdac1 启动子 CpG 岛的甲基化模式在比较玻璃化卵母细胞和新鲜卵母细胞时没有统计学意义。比较玻璃化卵母细胞和新鲜卵母细胞时，Hat1 和 Hdac1 mRNA 的表达水平没有统计学差异。Dnmt1o mRNA 在玻璃化卵母细胞中的表达明显低于新鲜卵母细胞。

结论

玻璃化处理对 Dnmt1o、Hat1 或 Hdac1 启动子 CpG 岛的甲基化模式没有统计学意义的改变，但明显降低了小鼠 MII 卵母细胞中 Dnmt1o mRNA 的表达。

相似文献

1
Effect of vitrification on promoter CpG island methylation patterns and expression levels of DNA methyltransferase 1o, histone acetyltransferase 1, and deacetylase 1 in metaphase II mouse oocytes.玻璃化对中期 II 期小鼠卵母细胞中启动子 CpG 岛甲基化模式以及 DNA 甲基转移酶 1o、组蛋白乙酰转移酶 1 和去乙酰化酶 1 表达水平的影响。
Fertil Steril. 2013 Jul;100(1):256-61. doi: 10.1016/j.fertnstert.2013.03.009. Epub 2013 Mar 30.
2
Effect of oocyte vitrification on deoxyribonucleic acid methylation of H19, Peg3, and Snrpn differentially methylated regions in mouse blastocysts.卵母细胞玻璃化冷冻对小鼠囊胚中 H19、Peg3 和 Snrpn 差异甲基化区域脱氧核糖核酸甲基化的影响。
Fertil Steril. 2014 Oct;102(4):1183-1190.e3. doi: 10.1016/j.fertnstert.2014.06.037. Epub 2014 Jul 23.
3
Dynamic methylation pattern of the methyltransferase1o (Dnmt1o) 5'-flanking region during mouse oogenesis and spermatogenesis.在小鼠卵子发生和精子发生过程中，甲基转移酶 1o（Dnmt1o）5'-侧翼区的动态甲基化模式。
Mol Reprod Dev. 2013 Mar;80(3):212-22. doi: 10.1002/mrd.22153. Epub 2013 Feb 28.
4
Histone deacetyltransferase1 expression in mouse oocyte and their in vitro-fertilized embryo: effect of oocyte vitrification.组蛋白去乙酰化酶1在小鼠卵母细胞及其体外受精胚胎中的表达：卵母细胞玻璃化的影响
Cryo Letters. 2011 Jan-Feb;32(1):13-20.
5
Differential methylation of pluripotency gene promoters in in vitro matured and vitrified, in vivo-matured mouse oocytes.体外成熟和玻璃化冷冻、体内成熟的小鼠卵母细胞中多能性基因启动子的差异甲基化。
Fertil Steril. 2011 May;95(6):2094-9. doi: 10.1016/j.fertnstert.2011.02.011. Epub 2011 Apr 1.
6
Effect of 5-aza-2'-deoxycytidine on methylation of the putative imprinted control region of H19 during the in vitro development of vitrified bovine two-cell embryos.5-氮杂-2'-脱氧胞苷对玻璃化冷冻牛二细胞胚胎体外发育过程中 H19 假定印迹控制区甲基化的影响。
Fertil Steril. 2012 Jul;98(1):222-7. doi: 10.1016/j.fertnstert.2012.04.014. Epub 2012 May 22.
7
DNA methylation in ovarian cancer. II. Expression of DNA methyltransferases in ovarian cancer cell lines and normal ovarian epithelial cells.卵巢癌中的DNA甲基化。II. DNA甲基转移酶在卵巢癌细胞系和正常卵巢上皮细胞中的表达。
Gynecol Oncol. 2001 Aug;82(2):299-304. doi: 10.1006/gyno.2001.6284.
8
Cytokeratin distribution and expression during the maturation of mouse germinal vesicle oocytes after vitrification.玻璃化冷冻后小鼠生发泡期卵母细胞成熟过程中细胞角蛋白的分布和表达。
Cryobiology. 2013 Jun;66(3):261-6. doi: 10.1016/j.cryobiol.2013.02.062. Epub 2013 Mar 7.
9
Cell division is required for de novo methylation of CpG islands in bladder cancer cells.膀胱癌细胞中CpG岛的从头甲基化需要细胞分裂。
Cancer Res. 2002 Apr 15;62(8):2378-84.
10
Aberrant methylation and silencing of ARHI, an imprinted tumor suppressor gene in which the function is lost in breast cancers.ARHI基因（一种印记肿瘤抑制基因）的异常甲基化和沉默，该基因在乳腺癌中功能丧失。
Cancer Res. 2003 Jul 15;63(14):4174-80.

引用本文的文献

1
Embryo vitrification impacts learning and spatial memory by altering the imprinting genes expression level in the mouse offspring' hippocampus.胚胎玻璃化冷冻通过改变小鼠子代海马体中印迹基因的表达水平影响学习和空间记忆。
Sci Rep. 2025 Feb 13;15(1):5419. doi: 10.1038/s41598-025-89857-6.
2
Association of duration of embryo culture with risk of large for gestational age delivery in cryopreserved embryo transfer cycles.胚胎培养时间与冷冻胚胎移植周期中巨大儿分娩风险的关系。
Fertil Steril. 2024 May;121(5):814-823. doi: 10.1016/j.fertnstert.2024.01.002. Epub 2024 Jan 5.
3
Long-term health risk of offspring born from assisted reproductive technologies.
辅助生殖技术出生子代的长期健康风险。
J Assist Reprod Genet. 2024 Mar;41(3):527-550. doi: 10.1007/s10815-023-02988-5. Epub 2023 Dec 26.
4
Improvement of Fertilization Capacity and Developmental Ability of Vitrified Bovine Oocytes by JUNO mRNA Microinjection and Cholesterol-Loaded Methyl-β-Cyclodextrin Treatment.JUNO mRNA 显微注射和胆固醇负载的甲基-β-环糊精处理对玻璃化牛卵母细胞受精能力和发育能力的改善。
Int J Mol Sci. 2022 Dec 29;24(1):590. doi: 10.3390/ijms24010590.
5
Effects of oocyte vitrification on gene expression in the liver and kidney tissues of adult offspring.卵母细胞玻璃化冷冻对成年子代肝、肾组织基因表达的影响。
J Assist Reprod Genet. 2022 Nov;39(11):2635-2646. doi: 10.1007/s10815-022-02611-z. Epub 2022 Oct 12.
6
Long-Term Storage Does Not Affect the Expression Profiles of mRNA and Long Non-Coding RNA in Vitrified-Warmed Human Embryos.长期储存不影响玻璃化冷冻-复温后的人类胚胎中mRNA和长链非编码RNA的表达谱。
Front Genet. 2022 Feb 1;12:751467. doi: 10.3389/fgene.2021.751467. eCollection 2021.
7
Preserving Oocytes in Oncofertility†.在肿瘤生育力保护中保存卵母细胞。
Biol Reprod. 2022 Feb 22;106(2):328-337. doi: 10.1093/biolre/ioac008.
8
Cryopreservation of Gametes and Embryos and Their Molecular Changes.配子和胚胎的冷冻保存及其分子变化。
Int J Mol Sci. 2021 Oct 8;22(19):10864. doi: 10.3390/ijms221910864.
9
What impact does oocyte vitrification have on epigenetics and gene expression?卵母细胞玻璃化冷冻对表观遗传学和基因表达有什么影响？
Clin Epigenetics. 2020 Aug 10;12(1):121. doi: 10.1186/s13148-020-00911-8.
10
Vitrification of human immature oocytes before and after in vitro maturation: a review.人类未成熟卵母细胞玻璃化冷冻保存：综述。
J Assist Reprod Genet. 2017 Nov;34(11):1413-1426. doi: 10.1007/s10815-017-1005-4. Epub 2017 Aug 18.