Agriculture Genetic Engineering Research Institute (AGERI), ARC, Giza, Egypt.
GM Crops Food. 2013 Jan-Mar;4(1):67-73. doi: 10.4161/gmcr.24324. Epub 2013 Jan 1.
Bioregulators have a great effect on vital processes of plant growth and development. Known plant bioregulators include Naphthalene acetic acid (NAA), Indole-3-butyric acid (IBA) and Indole-3-acetic acid (IAA). Natural or synthetic plant bioregulators are organic compounds that affect the physiological processes in the plant, either to control some of these processes or to modify them. For example these bioregulators can affect the nature of the process, either by accelerating or decelerating plant growth, rates of maturation and also by altering the behavior of the plants or their products. Also, enhancement of important nutrients in human diet could be achieved by bioregulators. This study uses the model crop plant Tomato (Lycopersicon esculentum). Tomato is affected by a group of bioregulators, this group contains compounds which are powerful antioxidants in vitro. The current study aims to find out the effect of some plant bioregulators (IAA, IBA and NAA) on tomato growth, total protein content and enzyme activities of ascorbate peroxidase (APX), superoxide dismutase (SOD) and catalase (CAT). This study also investigates the effect of the above mentioned bioregulators on the level of RNA expression for SOD, CAT and TPX1 genes. The analytical quantification of target gene expression showed the induced effect of NAA on SOD expression and reducing effect of the other bioregulators (IAA and IBA) on CAT and TPX1 expression. However, at the protein level, we found that IBA and IAA caused a minor effect on total protein content while a significant effect was recorded on the total protein level using NAA. Upon measuring the enzyme activity of ascorbate peroxidase and catalase, we found that both the exogenous NAA and IBA stimulated ascorbate peroxidase activity in tomato while there was no considerable difference detected in IAA treated plants. Also, there was no considerable difference detected in catalase activity of all bioregulator-treated plants compared with the control.
生物调节剂对植物生长和发育的重要过程有很大的影响。已知的植物生物调节剂包括萘乙酸（NAA）、吲哚丁酸（IBA）和吲哚乙酸（IAA）。天然或合成的植物生物调节剂是影响植物生理过程的有机化合物，要么控制这些过程中的一些，要么改变它们。例如，这些生物调节剂可以影响过程的性质，无论是通过加速还是减缓植物的生长、成熟速度，还是通过改变植物或其产物的行为。此外，还可以通过生物调节剂来增强人类饮食中的重要营养物质。
本研究使用模式作物番茄（Lycopersicon esculentum）。番茄受到一组生物调节剂的影响，这组调节剂包含在体外具有强大抗氧化能力的化合物。本研究旨在研究一些植物生物调节剂（IAA、IBA 和 NAA）对番茄生长、总蛋白含量以及抗坏血酸过氧化物酶（APX）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的酶活性的影响。本研究还研究了上述生物调节剂对 SOD、CAT 和 TPX1 基因的 RNA 表达水平的影响。目标基因表达的分析定量表明，NAA 对 SOD 表达有诱导作用，而其他生物调节剂（IAA 和 IBA）对 CAT 和 TPX1 表达有抑制作用。然而，在蛋白质水平上，我们发现 IBA 和 IAA 对总蛋白含量有轻微影响，而 NAA 则显著影响总蛋白水平。在测量抗坏血酸过氧化物酶和过氧化氢酶的酶活性时，我们发现外源 NAA 和 IBA 均能刺激番茄中抗坏血酸过氧化物酶的活性，而在 IAA 处理的植物中则未检测到明显差异。此外，与对照相比，所有生物调节剂处理的植物中过氧化氢酶的活性没有明显差异。
