Bahramsoltani M, Slosarek I, De Spiegelaere W, Plendl J
Faculty of Veterinary Medicine, Institute of Veterinary Anatomy, University of Leipzig, An den Tierkliniken 43, 04103, Leipzig, Germany.
Anat Histol Embryol. 2014 Apr;43(2):103-15. doi: 10.1111/ahe.12052. Epub 2013 Apr 4.
In vitro angiogenesis assays constitute an important tool for studying the mechanisms of angiogenesis and for identification of pro- and anti-angiogenic substances. Therefore, endothelial cell and media systems used for in vitro angiogenesis assays are required to mimic the angiogenic process in vivo including endothelial capability to express collagen type IV as a component of the basement membrane. In this study, the expression of collagen type IV and its α chains (α1-6) was investigated in different endothelial cell culture systems in vitro qualitatively and quantitatively. These systems included four different batches of microvascular endothelial cells derived from the human skin, heart and lung, from which only two batches were found to be angiogenic and two batches were classified as non-angiogenic. Distribution of the transcripts of the α chains of collagen type IV was similar in all cell and media systems investigated. However, secretion and deposition of a stable extracellular network of collagen type IV could only be observed in the angiogenic cultures. In conclusion, the consecutive steps of the angiogenic cascade in vivo as well as in vitro depend on an increasing secretion and subsequent extracellular deposition of collagen type IV.
体外血管生成测定是研究血管生成机制以及鉴定促血管生成和抗血管生成物质的重要工具。因此，用于体外血管生成测定的内皮细胞和培养基系统需要模拟体内的血管生成过程，包括内皮细胞表达作为基底膜成分的IV型胶原的能力。在本研究中，对不同体外内皮细胞培养系统中IV型胶原及其α链（α1 - 6）的表达进行了定性和定量研究。这些系统包括来自人皮肤、心脏和肺的四批不同的微血管内皮细胞，其中只有两批具有血管生成能力，两批被归类为无血管生成能力。在所有研究的细胞和培养基系统中，IV型胶原α链转录本的分布相似。然而，只有在具有血管生成能力的培养物中才能观察到稳定的IV型胶原细胞外网络的分泌和沉积。总之，体内以及体外血管生成级联反应的连续步骤取决于IV型胶原分泌的增加及其随后在细胞外的沉积。
