Jarolim E, Poulsen L K, Stadler B M, Mosbech H, Oesterballe O, Kraft D, Weeke B
Medical Department TTA, State University Hospital, Copenhagen, Denmark.
J Allergy Clin Immunol. 1990 Jun;85(6):996-1004. doi: 10.1016/0091-6749(90)90043-4.
The formation of specific IgE, IgG1, and IgG4 antibodies was investigated by immunoblotting during hyposensitization with timothy grass-pollen extract and 6 years later, Until the end of immunotherapy, specific IgG antibody levels increased. Also simultaneously, the number of allergenic components detected by IgG increased. However, this IgG response was similar in responding and nonresponding patients; thus, it did not correlate with the clinical outcome of the therapy. More allergenic compounds were also detected by IgE on immunoblots, but again without correlation to success of therapy. Six years after immunotherapy, the therapeutic effect was still present, although by now the observed immunoglobulin-binding patterns were similar to patterns observed in the same patients' sera collected before the initiation of hyposensitization. Thus, changes of antibody-binding patterns in immunoblot do not relate to the success or failure of immunotherapy.
在用梯牧草花粉提取物进行减敏治疗期间及6年后，通过免疫印迹法研究了特异性IgE、IgG1和IgG4抗体的形成。直到免疫治疗结束，特异性IgG抗体水平升高。同时，由IgG检测到的变应原成分数量也增加。然而，这种IgG反应在有反应和无反应的患者中相似；因此，它与治疗的临床结果无关。在免疫印迹上也由IgE检测到更多的变应原化合物，但同样与治疗成功与否无关。免疫治疗6年后，治疗效果仍然存在，尽管此时观察到的免疫球蛋白结合模式与在减敏治疗开始前采集的同一患者血清中观察到的模式相似。因此，免疫印迹中抗体结合模式的变化与免疫治疗的成败无关。
